BIO LAB Worksheet Manip DNA

BIO LAB Worksheet Manip DNA - Manipulating DNA Worksheet...

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Manipulating DNA Worksheet Name Vivy Vu Section______ Date November 3, 2009 Exercise 1: Build a sequence of DNA Is each group’s DNA sequence of 5 base pairs (short for nucleotide pairs) the same or different? different How many variations of a sequence can take place with 5 base pairs? 2 pts 1024 possibilities If seven groups linked their sequences together, the simulated DNA would be 35 base pairs in length. Imagine how many variations could take place. One genes-worth of information can range from 100 to 1 million nucleotide pairs of DNA. Each gene has a distinct, constant sequence of nucleotide pairs. The sequence is what provides the cell with the information to make one specific protein. (The exact sequence is critical for the function of the gene. In sickle cell anemia, the abnormal hemoglobin gene has only one nucleotide pair that is different from the normal hemoglobin gene!) If the average gene is about 1000 base pairs long and there are only 4 different nucleotides ……. .than how can there be 30,000 different genes in the human genome? 2 pts crossing over, mutations, translocations Exercise 2: Restriction Enzymes cut DNA at specific sequences The cutting patterns of three different restriction enzymes are shown on the next page. A,T,G,C represent the four specific nucleotides in the recognition sequences and N are not specific and could be any nucleotide. Compare enzymes #1, #2, and #3 recognition site sequences to the base sequences on the bacterial plasmid DNA and
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BIO LAB Worksheet Manip DNA - Manipulating DNA Worksheet...

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