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Unformatted text preview: Core enzyme attaches and make RNA copy Sigma detaches and core enzyme finishes the job • Prokaryotic Transcription Initiation o Promoters locations, where is starts, = the promoter area Sigma recognizes these spots Sigma initiates the transcription process then detaches -35 box • Pribnow box • TTGACA • 35 letters up -10 box • Tata box • TATAA • 10 letters up o sigma opens the -10box promoter and pulls it into the core enzyme area DNA separates to make RNA copy o Mg region identifies the region where the transcription is taking place o Downstream DNA is never transcribed • Transcription elongation and Termination o Elongation Continue to read the DNA Transcribe a piece of mRNA (growing RNA strand) Reaches a termination sequence o Termination Know it’s the end of gene RNA pulls off DNA closes up again • Reading and assembling DNA direction (3’ and 5’) is important to know •...
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- Fall '09
- DNA, RNA copy, core enzyme