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Unformatted text preview: Core enzyme attaches and make RNA copy Sigma detaches and core enzyme finishes the job Prokaryotic Transcription Initiation o Promoters locations, where is starts, = the promoter area Sigma recognizes these spots Sigma initiates the transcription process then detaches -35 box Pribnow box TTGACA 35 letters up -10 box Tata box TATAA 10 letters up o sigma opens the -10box promoter and pulls it into the core enzyme area DNA separates to make RNA copy o Mg region identifies the region where the transcription is taking place o Downstream DNA is never transcribed Transcription elongation and Termination o Elongation Continue to read the DNA Transcribe a piece of mRNA (growing RNA strand) Reaches a termination sequence o Termination Know its the end of gene RNA pulls off DNA closes up again Reading and assembling DNA direction (3 and 5) is important to know...
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- Fall '09