Chapter 20 - DNA Technology and Genomics

Chapter 20 - DNA Technology and Genomics - Chapter 20 Class...

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Chapter 20 Class Notes – DNA Technology and Genomics – Page 1 Max Sauberman AP Biology – Mr. Schilp Chapter 20 DNA Technology and Genomics Understanding and Manipulating Genomes: Sequencing of the human genome was largely completed by 2003. DNA sequencing has depended on advances in technology, starting with making recombinant DNA. In recombinant DNA, nucleotide sequences from two different sources are combined in vitro into the same DNA molecule. Methods for making recombinant DNA are central to genetic engineering, the direct manipulation of genes for practical purposes. DNA technology has revolutionized biotechnology, the manipulation of organisms or their genetic components to make useful products. An example of DNA technology is the microarray, a measurement of gene expression of thousands of different genes. DNA Cloning: DNA cloning permits the production of multiple copies of a specific gene or other DNA segment. Working directly with specific genes, scientists prepare gene-sized pieces of DNA in identical copies, a process called gene cloning. Most methods for cloning pieces of DNA in the laboratory share general features, such as the use of bacteria and their plasmids. Cloned genes are useful for making copies of a particular gene and producing a gene product. A gene is placed into a plasmid in bacteria and is cloned. Using Restriction Enzymes to make recombinant DNA: Bacterial restriction enzymes cut DNA molecules at DNA sequences called restriction sites.
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This note was uploaded on 03/17/2011 for the course BIO 101 taught by Professor Sullivan during the Spring '08 term at Harvard.

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Chapter 20 - DNA Technology and Genomics - Chapter 20 Class...

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