TLCnotesCHEM216W11-2

TLCnotesCHEM216W11-2 - Chem 216 W11 Notes Topic:...

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Chem 216 W11 Notes Topic : ___Thin-Layer Chromatography__ page 1 of 2. Thin-Layer Chromatography (TLC) ( see: Pavia, pp 777-791). I. TLC - A highly sensitive analytical technique used to: (1) Determine the purity of a sample (# of components in a mixture) (2) Determine the identity of two compounds (3) Monitor the progress of a reaction •Requires only microgram or γ (10 -6 grams!) quantities of a sample. •Every reaction to be performed in the lab should be monitored by TLC. II. TLC plates : glass plates coated with silica gel that is impregnated with a fluorescent dye (use a 254 nm UV lamp for detection of the spots). co-spot (i.e., A and B) solvent front A B d 0 d A d 0 travel distance of the solvent d A travel distance of compound A R f (ratio to front) value for A = d A / d 0 • By definition, Rf values are between 0 and 1.0. • Best resolution is achieved in the Rf 0.3 - 0.7 range. • Make sure to dissolve only a mall amount of a smaple (2-3 mg) in a small volume of solvent (usually 0.5 - 1.0
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This note was uploaded on 03/19/2011 for the course CHEM 216 taught by Professor Nolta during the Spring '07 term at University of Michigan.

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TLCnotesCHEM216W11-2 - Chem 216 W11 Notes Topic:...

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