Lecture11 - Lecture 11 Lecture 11.13 The Formation of Hfr Strains and Chromosome Mobilization 11.14 Complementation 11.16 Mobile DNA Transposable

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Lecture 11 Lecture 11 11.13 The Formation of Hfr Strains and Chromosome Mobilization 11.14 Complementation 11.16 Mobile DNA: Transposable Elements 11.1 Genetic Map of the Escherichia coli Chromosome
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transfer of plasmid DNA by conjugation transfer of plasmid DNA by conjugation:
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contact between two conjugating bacteria: contact between two conjugating bacteria: initiated via pilus retraction of pilus within donor cell draws two cells together transfer of DNA occurs once mating pair drawn together F + cell fimbriae (sex pilus)
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replication and transfer process: replication and transfer process:
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11.13 The Formation of Hfr Strains and Chromosome Mobilization F plasmid is an episome; can integrate into host chromosome Cells possessing a non-integrated F plasmid are called F+ Cells possessing an integrated F plasmid are called Hfr (h igh f requency of r ecombination) High rates of genetic recombination between genes on the donor chromosome and those of the recipient
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integration of F plasmid into chromosome: integration of F plasmid into chromosome:
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breakage of Hfr chromosome at OriT beginning of transfer to F- recipient
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different Hfr strains different Hfr strains: circular bacterial chromosome can open at various insertion sequences at which the F plasmids become inserted
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This note was uploaded on 03/19/2011 for the course BIOL 140 taught by Professor Dr.joshneufeld during the Spring '10 term at Waterloo.

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Lecture11 - Lecture 11 Lecture 11.13 The Formation of Hfr Strains and Chromosome Mobilization 11.14 Complementation 11.16 Mobile DNA Transposable

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