2009_iClicker_W_-_Post-genomic_analysis

2009_iClicker_W_-_Post-genomic_analysis - cDNAs on a gene...

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iClicker Questions Chapter 14 – Post-genomic analysis (2 questions)
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(1) In subtractive hybridization, the single stranded driver (control) and tester (treatment) cDNA hybrids are removed, leaving only the unique tester cDNA sequences from the two mRNA populations. Which is provided in excess to make sure that all the potential driver-tester hybrids are eliminated? A. Driver cDNA B. Tester cDNA C. Both Driver and Tester cDNA
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GENE ARRAY TECHNOLOGY Quantitation of the differential expression in transcriptomes of control and treatment tissues/conditions involves the differential hybridization of the
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Unformatted text preview: cDNAs on a gene array, consisting of PCR products or oligonucleotides (Affymetrix). A. Upregulation of the gene mRNA transcript in the tumor B. Downregulation of the gene mRNA transcript in the tumor C. No change in expression between normal and tumor tissue D. All of the above is true E. None of the above is true (2) In the following gene array, normal breast tissue is compared to breast tumor tissue. The normal tissue cDNA is labeled with green (Cy3) and the tumor tissue cDNA is labeled with red dye (Cy5). What does a red dots mean?...
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2009_iClicker_W_-_Post-genomic_analysis - cDNAs on a gene...

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