2009_iClicker_S_-_sequencing

2009_iClicker_S_-_sequencing - 3. Second strand synthesis...

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iClicker Questions Chapter 9 – Sequencing (1 question)
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(1) Nucleotide strands are synthesized in the following techniques. Link the technique with the type of nucleotide strand produced A. 1-I, 2-II, 3-III, 4-IV, 5-V B. 1-II, 2-I, 3-III, 4-IV, 5-V C. 1-II, 2-I, 3-III, 4-V, 5-IV D. 1-II, 2-III, 3-I, 4-V, 5-IV E. None of the above is true TECHNIQUE 1. 5’ RACE 1. Cycle sequencing (Sanger chain- termination method) 2. Reverse-transcription of mRNA
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Unformatted text preview: 3. Second strand synthesis 4. Transcription Nucleotide strand produced I. Single stranded DNA of differing lengths (primed from one DNA primer) II. DNA amplification in the PCR (involves two DNA primers) III. Single-stranded cDNA (primed from a DNA primer) IV. Creation of a double-stranded cDNA from a single-stranded cDNA (primed with single DNA primer) V. A single mRNA (primed from a single DNA primer)...
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This note was uploaded on 03/19/2011 for the course BIOL 208 taught by Professor Chuong during the Spring '09 term at Waterloo.

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2009_iClicker_S_-_sequencing - 3. Second strand synthesis...

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