2009_iClicker_R_-_PCR

2009_iClicker_R_-_PCR - the gene using RACE or Rapid...

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iClicker Questions Chapter 8 – PCR (2 questions)
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(1) You analyse the qPCR data and compare the mRNA transcript expression (template is first strand cDNA) of Genes A and B and C. Which of the following is true? A. By PCR cycle 30, the fluorescence signal is equal for A, B and C. Therefore, the mRNA transcript levels for A and B and C are the same. B. Gene B is 4 times more abundant than Gene A C. Gene A is 32 times more abundant than Gene C D. Gene C is 8 times more abundant than Gene B E. None of the above is true
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(2) You have isolated a partial gene that codes for the middle part of the human growth hormone receptor gene one (HGR1). You want to isolate the 5’ and 3’ ends of
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Unformatted text preview: the gene using RACE or Rapid Amplification of cDNA Ends. You design a gene specific primer (GSP) in the middle and a universal primer at the end of the gene. What two primers do you use for 5 and 3 RACE? TECHNIQUE 1. 5 RACE 2. 3 RACE 5 3 3 5 Gene specific primer A. Antisense primer S. Sense primer DNA that hybridizes for anchor primer H. Complementary to homopolymeric tailed sequence added by terminal transferase T. Oligo-dT or (TTTTTTTTTTTTTT) A. 1-A-H, 2-S-T B. 1-S-H, 2-A-T C. 1-A-T, 2-S-H D. 1-A-T, 2-S-T E. Only one primer is needed Partial gene: Full length gene:...
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This note was uploaded on 03/19/2011 for the course BIOL 208 taught by Professor Chuong during the Spring '09 term at Waterloo.

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2009_iClicker_R_-_PCR - the gene using RACE or Rapid...

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