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2009_iClicker_N_-_libraries

2009_iClicker_N_-_libraries - one clone that hybridizes to...

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iClicker Questions Chapter 6 – Genomic and cDNA Libraries (2 questions)
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(1) What is not true about genomic libraries: A. Mechanical shearing or partial digest of the DNA ensures that the average insert sizes are small fraction of the size of the chromosome B. You need to screen only the size of the genome (=average insert size/clone x number of clone) to have a 99% chance of finding a particular gene in the genome. C. Most genomic libraries are often made in phage than bacteria because larger insert sizes can be accommodated in phage and it is easier to screen millions of clones by filter hybridization in a single experiment D. If you screen a genomic library, you are likely to find more than
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Unformatted text preview: one clone that hybridizes to your probe E. All the above statements are true (2) Which of the following treatment or reverse primers could be used to enrich the synthesis of the 5’ end of cDNA? A. Prime the synthesis of the first strand of cDNA using oligo-dT primer B. Prime the synthesis of the first strand of cDNA using random hexamers (NNNNNN) where N = dNTP C. Homopolymeric tailing the 5’ end of the first strand of cDNA with dCTP, and prime the second strand synthesis with an oligo-dG primer D. Allowing natural hairpin loop of single stranded cDNA to prime the synthesis of the second strand of cDNA E. B or C...
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