2009_iClicker_O_-_Finding_the_Right_Clone

2009_iClicker_O_-_Finding_the_Right_Clone - (Dig Z Enzyme...

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iClicker Questions Chapter 7 – Finding the Right Clone (2 questions)
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(1) Which of the following is not true? 1. An expression libraries contain promoter elements that permit conditional protein expression of all the clones in a cDNA library in the presence of an inducer (such as IPTG) 1. A goat anti-rabbit secondary antibody will bind to a primary antibody raised in rabbits 2. An enzyme-linked secondary antibody will drive a reaction to produce a color precipitate or light 3. A polyclonal antibody will recognize just one epitope on the antibody 4. Multiple banding pattern in a lane Western blot suggests different isoforms of proteins are expressed
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(2) Probes are created by substituting nucleotides with a detectable label. Link the type of probe with the detection process X. Radio-labelled probes ( 32 P) Y. Enzyme linked chromogenic reaction
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Unformatted text preview: (Dig) Z. Enzyme linked chemiluminescence (eg. horseradish peroxidase) 1. Radioactive decay of unstable atomic nucleus loses energy by emitting high energy beta particles which are detected on X-ray film or phosphoimager 1. light emited upon breakdown of product can be detected by X-ray film or phosphoimager 2. Forms a visible color precipitate A. X-1, Y-2, Z-3 B. X-1, Y-3, Z-2 C. X-2, Y-1, Z-3 D. X-2, Y-3, Z-1 E. X-3, Y-2, Z-1 (1) One of two primer sets (both 20 bp in length) for the PCR has a melting curve shifted to the left (2) compared to the other pair (1). What is responsible for the leftward shift in the melting curve and lower Tm for primer set 2? A. Primer set 2 has higher GC content B. Primer set 2 has higher AT content C. None of the above 1 2...
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2009_iClicker_O_-_Finding_the_Right_Clone - (Dig Z Enzyme...

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