2009_iClicker_J_-_Chapter_4

2009_iClicker_J_-_Chapter_4 - iClicker Questions Chapter 4...

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iClicker Questions Chapter 4 – Cutting and Joining DNA (6 questions)
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(1) Does PstI produce a 5’ or 3’ overhang, given that its recognition sequence is CTGCA/G? A. 5’ overhang B. 3’ overhang C. Too early, too sleepy D. Don’t care E. Why do I need to know this?
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(2) What are the cut ends from a restriction digest using BamHI of the sequence: 5’ ATGAGGATCCAGTT 3’, knowing that BamHI recognizes G/GATCC A. (1) and (6) B. (2) and (4) C. (2) and (5) D. (3) and (6) E. (1) and (5) Enzyme Recognition site # of bases BamHI G /GATCC 6 BglII A/GATCT 6 CAGTT CTAGGTCAA GATCCAGTT GTCAA ATGAG TACTCCTAG TACTC ATGAGGATCC ATGA TACTGGATCC GGATCCAGTT TCAA (1) (2) (3) (4) (5) (6)
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(3) Which of the following two pairs of enzymes in a double digest would produce compatible, sticky ends that would ligate together? A. XmaI and SmaI B. BamHI and BglII C. BamHI and EcoRI D. BamHI and KpnI E. Acc65I and KpnI
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The dephosphorylated vector will not religate on itself with the missing phosphate groups. Where are the phosphate(s) that are removed on the vector?
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This note was uploaded on 03/19/2011 for the course BIOL 208 taught by Professor Chuong during the Spring '09 term at Waterloo.

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2009_iClicker_J_-_Chapter_4 - iClicker Questions Chapter 4...

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