Lab Report - The Effects of Temperature and pH on Rate of...

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The Effects of Temperature and pH on Rate of Enzyme Reaction Alexa Graves BSC2010c Section:21 Seat:6 February 23, 2011 By signing below, I guarantee that I am the sole author of this laboratory report: ______________________________ ______________________ Name Date
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Introduction: Enzymes are a very common form of a catalyst, a component of chemical reaction that speeds up the rate of reaction without being destroyed by the reaction itself (Campbell et al. 2008). Each type of enzyme is built for a specific substrate (Campbell et al. 2008). The substrate binds to its specific enzyme at the active site, granted there are no competitive or non- competitive inhibitors present, and then the enzyme goes about breaking the substrate down (Campbell et al. 2008). Many factors may contribute to the speed of reaction (Campbell et al. 2008). The two factors that I tested are temperature and pH. Each enzyme has a specific temperature and pH in which it functions best (Thomas at al. 2011). In this experiment I used a species of bovine catalase, an enzyme normally found in the stomach of cows. Bovine catalase insistently binds NAPD (Fita and Rossman1985). The human’s normal body temperature is 37 ° C (Campbell et al. 2008). If this temperature is raised only a few degrees the human’s life is in jeopardy yet, lowered by a few degrees they will be fine (Campbell et al. 2008). I believe that the same reaction will occur in this experiment with the bovine catalase. My null hypothesis for the temperature part of this experiment is that the change in temperature settings will have no effect on the rate of chemical reaction of the bovine catalase. My alternative hypothesis for the temperature part of this experiment is that a room temperature will allow the bovine catalase to function best and the hotter temperature will inhibit the catalase reaction the most. Null (H 0 ): The temperature changes will not affect the rate of bovine catalase reaction. Alternative (H a ): The room temperature flask will have the most productive rate of catalase reaction, and the hotter temperature will have the most inhibited rate of reaction, leaving the ice
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bathed flask in the middle. Because catalase is usually found in the stomach (Campbell et al. 2008), I would hypothesize that a more acidic pH would be ideal for rate of reaction. My null hypothesis for the pH part of this experiment is that the change in pH will have no effect on the rate of catalase reaction. My alternative hypothesis for the pH part of this experiment is that the more acidic pH will have the best rate of reaction and the more basic pH will inhibit the catalase reaction the most. Null (H
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This note was uploaded on 03/21/2011 for the course BIO 2010 taught by Professor Thomas during the Spring '11 term at University of Central Florida.

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Lab Report - The Effects of Temperature and pH on Rate of...

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