F08 LS1a Pset 10final

F08 LS1a Pset 10final - Name: TF Name: 1 LS1a Problem Set...

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Unformatted text preview: Name: TF Name: 1 LS1a Problem Set #10 and Lab #9 Post-Lab Question Both assignments due Monday 12/15 at noon in your TF’s drop box on the 2 nd floor of the Science Center (100 problem set points + 30 lab points) Lab 9 post-lab question (30 points) : 1) Briefly summarize the results you obtained for each strain of bacteria tested in the lac operon lab. Based on your results, what is the identity of each strain? Name: TF Name: 2 LS1a Problem Set #10 Due Monday 12/15 at noon in your TF’s drop box on the 2 nd floor of the Science Center 1. (24 points) The transcription of the lacZ gene in E. coli bacteria is regulated in part by the binding of the lac repressor protein to the lac promoter. When bacteria are grown in media containing only glucose and no lactose, no β-galactosidase enzyme is produced. When bacteria are grown in media containing only lactose, increased levels of β-galactosidase enzyme are made. a. Briefly explain what accounts for the lactose-dependent expression of the lacZ gene in E. coli . b. The graph below shows an experiment performed by Jacques Monod during his studies of the lac operon. The left y-axis of the graph shows the number of bacteria that are present over time in a culture containing equimolar amounts of glucose and lactose. The right y-axis plots the levels of β-galactosidase enzyme activity over time. Explain what accounts for the lag between the first and second phase of bacterial growth. Name: TF Name: 2 LS1a Problem Set #10 Due Monday 12/15 at noon in your TF’s drop box on the 2 nd floor of the Science Center 1. (24 points) The transcription of the lacZ gene in E. coli bacteria is regulated in part by the binding of the lac repressor protein to the lac promoter. When bacteria are grown in media containing only glucose and no lactose, no β-galactosidase enzyme is produced. When bacteria are grown in media containing only lactose, increased is produced....
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F08 LS1a Pset 10final - Name: TF Name: 1 LS1a Problem Set...

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