F08 LS1a Pset 9 final

F08 LS1a Pset 9 final - Name: TF Name: LS1a Problem Set #9...

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Name: TF Name: 1 LS1a Problem Set #9 Due Monday 12/8 at noon in your TF’s drop box on the 2 nd floor of the Science Center (100 points) 1. (26 points) The structure of HIV protease is stable from pH 3-7; however, the activity of the enzyme varies within this range as shown below. a) Based on your understanding of catalysis by HIV protease, explain this relationship between protease activity and pH. You can assume that the tertiary and quaternary structures of the enzyme remain relatively stable between pH 3 and 7. b) What would be the consequence of mutating the catalytic Asp 25 into Asn? Assume substrate binding and protease structure are not affected by the mutation. c) What would be the consequence of mutating the catalytic Asp 25’ to Asn? Assume substrate binding and protease structure are not affected by the mutation. d) What would be the consequence of mutating Ile 50 to Gly? Assume substrate binding and protease structure are not affected by the mutation. Protease activity pH 3 4 5 6 7
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Name: TF Name: 2 2. (36 points) The emergence of multidrug-resistant bacteria has been attributed in
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F08 LS1a Pset 9 final - Name: TF Name: LS1a Problem Set #9...

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