F08 LS1a Pset 7final

F08 LS1a Pset 7final - Name: TF Name: 1 LS1a Fall 08...

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Unformatted text preview: Name: TF Name: 1 LS1a Fall 08 Problem Set #7 Due Monday 11/17 at noon in your TFs drop box on the 2 nd floor of the Science Center (100 points total) 1. (15 points) Scientists often clone eukaryotic genes and express them in prokaryotes like the bacterium E. coli . A genomic copy of the human gene encoding XXX is cloned into E. coli and you study the results. a) After expressing the gene in cultured prokaryotic and eukaryotic cells, the expected protein is affinity purified using a purification column. You run a protein gel and observe the following results. Give one possible explanation for the results seen on the gel, assuming that no problems were encountered during the purification. To determine the source of the difference, total RNA is purified from both the prokaryotic and eukaryotic cells and loaded onto an RNA gel. The gel is then analyzed specifically for the human gene and the following results are observed: Name: TF Name: 2 b) Why are there two major bands seen in the eukaryotic cells? c) Propose a plausible explanation of why there is only one band in the prokaryotic cells, and how this could result in the observation seen on the protein gel. The Genetic Code (for question 2): Name: TF Name: 3 2. (35 points) Below is the genomic DNA sequence of the human histone gene, H2B. The TATA box and the transcriptional start site are highlighted in bold . The middle sequence of the gene (131-342) is denoted by and not shown. This gene does not contain any introns. not contain any introns....
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F08 LS1a Pset 7final - Name: TF Name: 1 LS1a Fall 08...

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