LS1a_Oct_28_slides

LS1a_Oct_28_slides - LIFE S CI ENC 1a ES PROF. ROBERT LUE 1...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
PROF. ROBERT LUE OCTOBER 28, 2008 1a L I F E S C I E N C E S 1 Nucleic acids and the chemistry of replicating information II 1. The molecular basis of DNA replication - Semiconservative DNA replication - Nucleotide addition to a growing DNA strand - Is DNA polymerization energetically favorable? - The role of pyrophosphate cleavage 2. DNA replication in the cell - The DNA replication fork - DNA polymerase and the accuracy of replication 3. The Polymerase Chain Reaction - DNA amplification by PCR 2
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
DNA Polymerase is needed to synthesize a new strand of DNA O P O P O O O O O A T G G T - A C - G A - T G - C A T G G - C T - A C - G A - T G - C dCTP + Very slow (no observable reaction) O P O P O O O O O A T G G T - A C - G A - T G - C A T G G - C T - A C - G A - T G - C dCTP + Fast (~50 bases added per second) DNA polymerase Polymerase adds on to the 3’-OH Incoming deoxynucleotides must pair with template Requires primer to initiate 3 DNA Replication Both daughter strands must be synthesized in a 5’ to 3’ direction Leading strand synthesized contiguously Lagging strand initially made as a series of fragments 4
Background image of page 2
Other proteins play key roles in DNA replication 5 DNA replication is extremely accurate Selectivity based on hydrogen bonding alone: Method Error Rate ~1 in 100 With DNA polymerase: ~1 in 100,000,000 With all cellular machinery: ~1 in 10,000,000,000 6
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
How is incorporation of mismatched bases disfavored?
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 11

LS1a_Oct_28_slides - LIFE S CI ENC 1a ES PROF. ROBERT LUE 1...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online