Enzyme Report Student Instructions 2010

Enzyme Report Student Instructions 2010 - Student...

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Student Information for the Enzyme lab report You have two weeks to complete this report. It is due the week of 11/01 at your scheduled lab. Don’t put off doing the report until the last minute or you will regret it. The lab is worth 30 points. Review the lab manual for the basic report format. This information is specific to the enzyme lab. Attach the cover sheet that is posted on Blackboard. 1. Title : This should include the important information about the experiment that you conducted, and should not be simply (“lab report”, or the title of the lab). What enzyme are you using? 2. Introduction : Be sure to include: What is an enzyme? State your question to test. What experiments were conducted? How can you cover this in a sentence or two? Leave the details for discussion. Separate 3. Materials and Methods (protocol) : see lab manual for directions. You should include any deviations you made from the instructions in the manual. Include protocol from the graded worksheet you did during the previous lab if you have it or a copy, now with filled in tables. Since the exercise was done as a bench, you need a copy. You do not need to replicate the protocol in the M & M section, just refer to it – use page numbers and include changes. 4. Results : Remember to label all tables and graphs. If you use Excel, you can do it all on one sheet. See the next part for details on the presentation of data. Note that all the data goes in a single table or tables organized so that the data can be easily prepared. Graphs follow the table(s) Part B ; STD Curve: include a table of the O.D. vs. concentration for the standards. Plot data, and draw best fit line using Excel. You can then use Excel to help you plot all the data for the subsequent graphs. Note that while Excel can give you a trend line for the linear graphs, you may not want to use it. See the instructions for finding the initial velocity for enzyme activity in the lab manual. Part C; Rate of Reaction: Include a table of time, O.D. & [P] use the standard curve by equation or hand to convert O.D. to concentration of product [P]. Plot the [P] / Time. Draw the best-fit line by eye or use Excel. Determine enzyme activity or initial velocity (see lab manual for details). Include this in a table for rates of each part or on the graph. Part D ; Enzyme Concentration: Table of time, O.D. and [P] for all 5 enzyme concentrations, use the standard curve to convert O.D. to [P] as in B. Plot [P] v. time. Compare the line and equation Excel give you to initial velocity. From your measure of initial velocity, plot enzyme activity v. concentration of enzyme on a separate graph. Part E
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This note was uploaded on 03/27/2011 for the course BIO 2912 taught by Professor Waring/rappaport during the Spring '11 term at Temple.

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Enzyme Report Student Instructions 2010 - Student...

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