Chapter 20 Study Test - CHAPTER 20 AP BIOLOGY MULTIPLE...

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CHAPTER 20 AP BIOLOGY MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 1) It is theoretically possible for a gene from any organism to function in any other organism. Why is this possible? A) All organisms have the same genetic code. 2) One important approach to gene cloning uses B) plasmids. D) bacteria. 3) Current applications of gene cloning include A) cleaning up toxic waste. B) manufacturing human growth hormone. C) instilling pest resistance in plants. 4) Plasmids are important in biotechnology because they are A) a vehicle for the insertion of recombinant DNA into bacteria. 5) If you discovered a bacterial cell that contained no restriction enzymes, which of the following would you expect to happen? D) The cell would be easily infected and lysed by bacteriophages. 6) Which enzyme was used to produce the molecule in Figure 20.1? A) a restriction enzyme 7) Assume that you are trying to insert a gene into a plasmid and someone gives you a preparation of DNA cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to C) cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme. 8) What is the genetic function of restriction enzyme? A) cleaves nucleic acids at specific sites 9) The restriction enzyme used in constructing hybrid molecules of certain gene sequences and plasmid DNA acts by D) opening DNA molecules at specific sites, leaving sticky ends exposed. 10) How does a bacterial cell protect its own DNA from restriction enzymes? D) by adding methyl groups to adenines and cytosines 11) What two enzymes are needed to produce recombinant DNA? D) restriction enzyme, ligase 12) What is a cloning vector? A) an agent, such as a plasmid, used to transfer DNA from an in vitro solution into a living cell 13) What are the essential characteristics of a cloning vector? B) Bacterial cells replicate it. C) Bacterial cells take it up. I. Transform bacteria with recombinant DNA molecule II. Cut the plasmid DNA using restriction enzymes
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CHAPTER 20 AP BIOLOGY III. Extract plasmid DNA from bacterial cells IV. Hydrogen-bond the plasmid DNA to nonplasmid DNA fragments V. Use ligase to seal plasmid DNA to nonplasmid DNA 14) From the list above, which of the following is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium? A) III, II, IV, V, I 15) Bacteria containing recombinant plasmids are often identified by which process? A) exposing the bacteria to an antibiotic that kills the cells lacking the plasmid
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Chapter 20 Study Test - CHAPTER 20 AP BIOLOGY MULTIPLE...

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