ANS18-10-L09-ALGAE-SEAWEEDS

ANS18-10-L09-ALGAE-SEAWEEDS - Algal Culture unicellular and...

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Algal Culture – unicellular and multicellular (seaweeds) Total Plant Aquaculture = 15,781,000 mt Unicellular or micro-algal production - ? Multicellular – seaweeds = 13,241,000 mt 84% of total plant aquaculture
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Culture conditions necessary for growth of algae and seaweeds A. Light B. Carbon dioxide C. Appropriate temperature D. Nutrients E. Salinity F. Mixing Photosynthesis LIGHT ENERGY Carbon dioxide + water { CHLOROPHYLL } Oxygen + Sugar 12 (CO 2 + H 2 O) { CHLOROPHYLL } O 2 + C 6 H 12 O 6
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Phytoplankton (micro-algae) are microscopically small single-celled plants free floating in the water. Diatoms are a special group of algae with cell walls made of silicate. Some species use flagella or cilia to move within the water. Isochrysis= 5 microns in diameter Chaetoceros= 6 microns in diameter Note: the diameter of a human hair = 40 – 120 microns
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4 *Monchrysis lutheri 5 *Isochrysis galbana, (T.ISO) 5.5 *Thalassiosira pseudonana(3H) 8.5 *Tetraselmin suecica 5 Phaeodactylum tricornutum 6.5 Dunaliella tertiolecta 6 *Chaetoceros gracilis 2 Nannochloris occulata 2.5 *Chaetoceros calcitrans 7 Chlamydomonas coccoides 6 *Skeletonema costatum Size μ Flagellates Size μ Diatoms Table 1. Algal species commonly used as food in aquaculture. Those marked with an asterisk are most often fed to oysters and clams.
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Provasoli-Guillard National Center for Culture of Marine Phytoplankton CCMP's L/20 Medium Sterilize 1.0 L filtered seawater in teflon bottle. After the seawater has cooled, aseptically add the following stock solutions. (see recipe) f/2 Vitamin Solution 0.05 mL (see recipe) f/2 Trace Metal Solution 0.1 mL (see recipe) Alkaline Soil Extract 5.0 mL 37.2 g/L dH 2 O Na 2 EDTA*2H2O 0.1 mL 26.8 g/L dH 2 O NH 4 Cl 0.1 mL 5.0 g/L dH 2 O NaH 2 PO 4 *H 2 O 0.1 mL 75.0 g/L dH 2 O Na NO 3 0.1 mL Stock Sol. Compound Quan. Adjust pH to 7.8-8.2 with HCl
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In a flask add two parts by volume distilled water (dH2O) to one part rich organic garden soil (containing no recent applications of chemical fertilizer or pesticides). Add 2-3 g NaOH/liter. Autoclave for 2 hours, and filter when cool. This concentrated extract is diluted 1:50 in dH2O for the final working stock. (Seawater may substituted for dH2O if the extract is to be used for marine species). 180.0 g/L
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This note was uploaded on 03/25/2011 for the course ANS 18 taught by Professor Mchenry during the Spring '11 term at UC Davis.

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ANS18-10-L09-ALGAE-SEAWEEDS - Algal Culture unicellular and...

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