NPB+112+11+syn+trans

NPB+112+11+syn+trans - Synaptic transmission Outline:...

Info iconThis preview shows pages 1–7. Sign up to view the full content.

View Full Document Right Arrow Icon
1 Synaptic transmission Outline: 1/11/11 – 1/13/11 I. Overview II. Synaptic transmission and vesicular theory of neurotransmitter release (Ch. 5) A. requires Ca++ B. vesicular theory (quantal) C. mechanism of release D. recycling III. Neurotransmitters and postsynaptic receptors (Ch. 6) A. Acetylcholine B. glutamate and GABA C. ligand-gated (ionotropic) vs. metabotropic (G-protein-coupled) D. neuropeptides Synapse! Fig. 1.3 a
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
2 Fig. 5.1 (D’hondt et al, BioEssays 31:953, 2009; Mese et al., J Invest Derm 127:2516, 2007) Connexin
Background image of page 2
3 Fig. 5.3 Ca++ free (replace with cadmium) TTX, TEA added Fig. 5.10
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
4 presynaptic Microinject Ca++ or buffer into presynaptic terminal Fig. 5.11 Fig. 5.6 Neurotransmitter is acetylcholine * Low Ca 2+ conditions *
Background image of page 4
5 In multiples of 0.4 mV Fig. 5.7 Box 4A Patch-clamp Can record current flowing through single ion channel i ~2 pA, corresponds to depolarization of ~ 0.4 μ V = effect of opening of single postsynaptic ion channel “Quantal” end-plate potential = 0.4 mV = 1,000 x 0.4 μV (1,000 ion channels) Normal end-plate potential= 40 mV = 100 x 0.4 mV = 100 “quanta” = 100,000 channels Animation: http://www.sinauer.com/ neuroscience4e/ animations4.1.html
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
6 0.4 V Neurotransmitter molecule Molecular (single receptor) 0.4 mV (1,000 receptor channels= 2,000 transmitter molecules)
Background image of page 6
Image of page 7
This is the end of the preview. Sign up to access the rest of the document.

This document was uploaded on 03/26/2011.

Page1 / 22

NPB+112+11+syn+trans - Synaptic transmission Outline:...

This preview shows document pages 1 - 7. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online