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Session 8 - Life Sci 2C03 Session 8 Session 8 Molecular...

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Life Sci 2C03 – Session 8 Session 8 – Molecular Tools (cont…) In Situ Studies 1. In situ = in the original location 2. Used to study specimen by taking slices 3. In Situ Hybridization 1. Uses labeled DNA or RNA probes to hybridize against a tissue to study the distribution of a particular DNA or RNA 2. Where a particular DNA is being expressed: slice put probes on incubate overnight probe will enter cell and hybridize techniques used to see where hybridization has occurred visual image produced 1. Immunohistochemistry 1. Uses antibody against specific proteins to study its distribution in a tissue 2. If no protein expression no antibody binding occurs so no expression seen 1. Autoradiography 1. Detecting a particular protein, RNA or DNA by radiolabelling 2. Find out how much of it is expressed Transgenic Techniques 1. Principles: 2. You are trying to use a viral vector to knockout a gene OR to introduce a new gene (knock in) 1. Transgenic strategy
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