MCB+102+Exam+Metabolism+Key+Spring+2010

MCB+102+Exam+Metabolism+Key+Spring+2010 - Name SID GSI Name...

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Unformatted text preview: Name _ SID: GSI Name: Section: M88 t02 Exam: Metabeiisrn April 1, 2010 Tetai points e 109 For this exam, use the abbreviations: ATP, ADP, Pi, FAD, FiViN, NAD, NADP, CoA. t. We have seen that the citric acid cycle is irreversible in animal celis. Totai 8 pts (A) Name the enzyme(s) that cataiyze the reactions of the citric acid cycle that are irreversible in these organisms. 2 gets ON : \ v/dnzm {tyre x. x H , I,“ E s: r [N ( \Q/Voz tea/mu, r (B) By contrast, certain photosynthetic anaerobic bacteria such as Chlorobim are able to reverse these reactions and use the cycle to synthesize cell building blocks from C02. Starting with on-ketoglutarate labeled in the carbonyl carbon, show which carbon(s) of cxalcacetate would be labeled in its formation by reversing the cycle. In your answer, include the structures of the beginning a~ketoglutarate and the *4} if}; a“? i f’ w Avg/cw i 3, 5001a é, CH Gwen i) v . I | x i i. _ ‘ e A m It He / been by iii-gt, n CCQOH A? (C) Continuing on the reversal path, which carbon(s) of succinage would be labeled? Name & [email protected]’% Sill"): GSE Name: Section: 2. The discovery that animals fix 002 opened new doors in our understanding of metabolism. Total to gate (A) Write the equation by which animal celis fix 002 to form a 04 acid with a biotin- dependent enzyme. lnclude structural formulas and names of the reactants, product(s) and participating enzyme. 4.5 pts (B) What is the function of this enzyme under conditions in which intermediates of the citric acid cycle are drained away for arnlnc acid btosynthesis. 2 pts gr r ‘A #436: . [ l. r} I; ‘ Ewan» , I i ‘ L25 i. “53 {xttcwlcw' CD] lad/‘6’ if“;ifl{jt_lgwlfgth;::;{““a? .5 .. i i Name J2; . SID: GS! Name: Section: 3, During this semester we have learned that glucose can follow one of two major catabolic paths. Total 5 pts. 2.x a -,- ;_. i R. g e mi committed enzyme of each pathway. 2 pts. i S;- .- tyrant,“ l KC) Narhe the coenzymes that are reduced in each pathway in (A). 2 pts. 5‘; 4. Fill sit the bianks. Total 5 pts. 1 pt each (A) Sketetal muscle cells obtain ATP‘s in the formation of lactate from gtucose via glycolysis. X} (B) Skeletal muscie cells obtain xi?! ATP’s from glycogen in glycolysis. (C) Skeletalmuscle cells obtain a total of lizfl‘itifm, ATP's from the combined operation of giycolysis, citric acid cycle and bxidative phosphorylatton. (D) Heart muscle celis obtain - ATP's from the combined operation of glycolysis, citric acid cycle and oxidative phosphoryiation. (E) Yeast ceils obtain ATP'S in the fermentation of glucose. Name Rat/r f sro: 5. We saw that a quincne functions in the synthesis of ATP from ADP and Pi in mitochondria. “total 6 his. (A) Name the quinone and describe in no more than 10 words the properties that enable it to perform this function? 3 pts. (G) How many ATP's does the function of the quincne add to the total ATP formed in NAD— or NADP~linked phosphorylation in each organetle per 2 electrons? 1 pt. @rzfiw ‘ 6. Indicate whether each of the following statements is True or False by writings"?1 or "F." Total 5 ptst pt each. (A) Plants synthesize giycogen in plastids by a pathway similar to liver. (8) WT“ Pyridoxai phosphate functions in decarboxyiation reactions. (C) E Lipoic acid is used by certain cells to synthesize pyruvate from acetyl~ CoA and 002. mn- afi‘fi—y (D) “r” 6-Phoshogluconate deydrogenase generates both NADH and NADPH. (E) E The binding change mechanism functions in the regulation of ATP synthesis. Name Anew”? Sit“): 68% Name Section ?. The electrons released in the initial stage oxidation of fatty acid oxidation enter the electron transport chain via a special enzymatic route. Total 9 pie, ' (A) identity the coenzymes (prosthetic groups) that make this possibte. 1 pt W FAD i re, g (8) Sketch a diagram designating the position at which the electrons enter the eiectron. transport chain retative to NADH, ubiquinone and cytochrome oxidase. Identify the reievant complexes. 3.5 pts r.» t 3‘ ' ' I - .Et-fé ‘r‘ ‘ (0) Name the enzyme of the citric acid cycle that resenfiibies‘hits c'oonterpiart in fatty acid oxidation in having a speciai portal of entry for electrons released in its tic groups. 1.5 pts i. (D) Sketch a diagram designating the position at which the eiectrons released by the enzyme in (C) enter the electron transport chain reiative to NADH and Ubiquinone. Identify the retevant complexes. 3 pts N to \ X .5 7..” Egg/0 "fit. 50 L ""1 . tot?“ \% tilt r": (2 “hm—"wan Mn! .i "K 9 ‘iffiC o in n Section: 8. Due to its toxicity, NH3 reieased from protein turnover in muscie is transported in the blood to the liver in the form of amino acids. “Total 1G pts. (A) Draw the structural formula and give the name of the amino acid that is derived from dtketoglutarate in the citric acid cycie. 2 pts y 4w 2- f/f ,, t It. 3" _ _ in: . . . (8) Write the equation for the release of NHgtrom the ammo aCld In (A). include the structure of the reactants and product(s)~and name of the participating enzyme and any coenzyme(s) in your answer. 4.5 pts fleets-i Mei-i i , t M t5"; )1 t C: 5'? :1"; ME ‘ i If] a y it a r "" -‘ t”? it H - ‘t i‘ “a” ’ i ,m i g- “1:: ‘“ i *2; .‘ , r .r T 5 i/ fly“ '0) [iravvflthea structure and give the name of the metabolite formed from the {N'Hg in (B) that feeds into the urea cycle. 2 pie iv?“ acid that provides nitrogen entering the urea cycle. t.5 pts t3; Name SID: GS! Name: Section: 9. Photosynthetic 002 assimitation and gluconeogenesis are simiiar in several respects. Tetai t1 pts. (A) The reductive steps of the two processes are similar. Write a single equation that fits the reaction for both processes, identifying differences where they exist. in your answer, use structurat formulas for substrates and products. Give the names of the participating enzyme‘(s) and abbreviation(s) of cofactor(s). Designate which reaction is photosynthetic and which is giuconeogeneic. " ‘ '° ' (A) 4 5333 ’i 9 , it _ -: hi ‘ i @ \. i L- ‘ m W" r . Czi;._"”ii ‘3‘“ ,, . .- (C) Name the functions upétréam from the reactioms) in (A) that ' produces pyrophosphate (Pi-Pi) in the reactions leading to the synthesis of glycogen. . \ pt” [/1 D i”) we. ) 1.0336? We ‘2ij a i' a“: “in. Humans on a starvation diet share certain metabolic features with type 1 diabetic patients. "Totai i0 pts - 2 pie each part (A) in both conditions, state how the citric acid cycle is disturbed, t .. a A l i" :3 -‘ w“: “i a l "P l 1 Max-m if; at Ft: d/‘Frfi/L/ir’l ear-ta.» if {i E“ w (l rt 2-" Ar (B) What is the primary metabolite(s) sent from the liver to the brain under these conditions? (C) What type 0f diet would lead to (A) in a healthy individual? "l fl'w'"? , M {W l l. r «g t in. w .._.a/‘ »---‘ t “- (D) What would be the metabolic fate of glyceroi in (A)? ' Cafe? W0 Lt. 60m V?” i a l to l . A, "v . (1‘ \ i i if 1.3-4} 3. J - ,rC/ xi» "/3- ‘ Oar/QMLJCD (as-46,7 if i Q, gap/«r16? m i »« k—ux’ (E) How do plants prevent a probiem such as that describe above for diabetic patients? - .. (g- i tat Let Section: H. We have seen that hormones trigger major changes in glycolysis and gluconeogenesis in animals. Total to gate (A) Diagram the sequence of events by which giucagon increases the breakdown of glycogen to glucose. include the name of the enzymes and the regulatory ' metebotites involved, if any. - 5: gag" fete is ‘ (9 W33“? {1’4 Cwa‘Cvt ' “W7 ‘ . . l l - ' flit/“H i 1 \ Ci ‘ fl) ! y‘ . '1. 1 ‘ ‘ I.’ (B)To which'ftts‘sue is most of the glucose formed in (A) transported in exercising competitive athletes? 1 pt V f} mmgctefi» (C)'Name the main metabolic product formed from giucose in the tissue in (B). 1 pt. ( 5 Law {L93 (E) Name the glucagon-triggered metabolite thatgreguiates both glycolysis and gluconeogenesis.‘ ' t 52 t" .._...._ V __ m r- ‘V: ,, a l; I «.1, is“ If sf 2 L} Name SID: GS] Name: Section: 12. We have seen this semester that the buildup of atmospheric 02 in the Earth’s I history led to revolutionary positive changes in cellular processes. Total 10 pts (A)There were, however, negative consequencesmior example, in 002 fixation via the Calvin-Benson cycle in photosynthetic aerobes. Write the equation for the reaction of the Calvin~8enson cycie that is adversely affected by 02. In your equation assume that the reaction takes place in the absence of 02. lnciude structural formulas of substrates, products and coenzyme(s) as appropriate. 4 (B) What are the carbon product(s) formed in the presence of pure 02? Give names and structurai formulas in our answer. 2 pts m. («1‘ iii/EQ) ganja If" ‘ a f I . _ i : iii a Pitt‘s-r” it tit t — is ‘3 _ kg; if; m; k W} 5, (C) Name a group: of piants that evolved to bypass this problem. 1 pt J i (Di 002 assimilation via the Calvin~Benson cycie employs enzymes shared with other pathways as weil as enzymes unique to the cycle. Name the enzymes that are only used in the Calvin~Benson cycle. 3 pts ...
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MCB+102+Exam+Metabolism+Key+Spring+2010 - Name SID GSI Name...

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