2010 Bio 317 Lecture 13

2010 Bio 317 Lecture 13 - LR (active) Monomer Dimer Dimer...

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Earl Sutherland, 1971 1) Identifed cAMP as generated in liver cells aFter exposure to adrenaline 2) Demonstrated cAMP added to liver cells caused same response as adrenaline.
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caffeine X
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NEM poisons AC enzyme by covalent modifcation. Forskolin stimulates enzyme.
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Sutherland’s Model
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Reconstitution of Adrenaline response demonstrates the necessity of adrenaline receptor. Red blood cells: have AR and AC Adrenal cortex cells: have no AR but have AC
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Response (cAMP levels) no AR low AR med AR high AR -Adrenaline +Adrenaline Receptor Activation R (inactive) -----> R (active) ----->
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Unformatted text preview: LR (active) Monomer Dimer Dimer Activation (response) Ligand binding Sutherlands Model Martin Rodbell, 1967 experimental genetics approach to isolate a cyc-mutant used S49 lymphoma cells selection criteria: 1. growth in presence of adrenaline 2. maintain receptors that bind adrenaline result : obtained cells that bound adrenaline but with no response. BUT, some of these still had a forskolin response! HOW??? Many of these cells had defective AC enzymes. Alfred Gilman adrenaline Gs G AR dimer Adenylate Cyclase...
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2010 Bio 317 Lecture 13 - LR (active) Monomer Dimer Dimer...

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