2010 Bio 317 Lecture 19

2010 Bio 317 Lecture 19 - PC12 cells Proteins 7 Neuronal...

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11/10/10 1 Neuronal Growth Factors / Neurotrophins Rita Levi-Montalcini shared Nobel prize with Stanley Cohen for her discovery of Nerve Growth Factor
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11/10/10 2 Levi-Montalcini and chick embryos Elmer Bueker and the sarcoma Dorsal root ganglia (DRG) - NGF + NGF The Assay NGF acts directly
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11/10/10 3 Purifcation oF the Factor A rich source oF Factor, helps…
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11/10/10 4 Proof of NGF action Does NGF stimulate proliferation? Victor Hamburger and the trophic factor hypothesis. 1. Did Rita and Stan purify the intended factor? 2. Does the factor have relevance? Primary Neuron Culture
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11/10/10 5 Does NGF infuence neuronal phenotype, or is phenotype intrinsic to the neuron?
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11/10/10 6 - NGF + NGF
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Unformatted text preview: PC12 cells Proteins 11/10/10 7 Neuronal Growth Factor Actions Proof of NGF Actions In Vivo 11/10/10 8 What is the signaling pathway for NGF?-NGF +NGF 11/10/10 9 ras NGF action requires Ras Trk oncogene (tropomyosin receptor kinase) 11/10/10 10 The Trk orphan receptor is expressed in neurons 11/10/10 11 Questions that were left answered: 1. How does an oncogene pathway mediate different effects in neurons? 2. Why do NGF and EGF actions differ in neurons? 3. Why do the EGF receptor (proto-oncogene) and the EGFR oncogene have different effects in neurons? 4. How does NGF mediate such a wide range of actions in neurons?...
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This note was uploaded on 03/28/2011 for the course BIO 317 taught by Professor Simonhalegoua during the Fall '08 term at SUNY Stony Brook.

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2010 Bio 317 Lecture 19 - PC12 cells Proteins 7 Neuronal...

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