2010 Bio 311 Lab E Master Standard

2010 Bio 311 Lab E Master Standard - and the "c"...

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polynomial regression Page 1 POLYNOMIAL REGRESSION AS A MEANS OF DETERMINING MOLECULAR WEIGH Directions: First save the file under another name and keep the untouched original file as a backup. Next enter the mm from the origin that your protein standards traveled. Stay within the boxed area since the graph reads only that area. A graph line with form automatically. (If the line is not "straight", remove the mm from the top and/or bottom bands.) BioRad #310007846 mm MW color 199.78 blue 122.24 magenta 85.98 green 40.47 violet 31.64 orange 17.16 red 7.26 blue An equation for the best fit will appear on the graph. The equation is in the form: Copy the "a" constant into the box next to a= below, then the "b" constant into the box next to b=
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Unformatted text preview: and the "c" constant into the box next to c= a= b= c= Record the mobility of the proteins in mm in the column marked "mm" . The Molecular Weight (MW) of the unknown protein will be calculated in the column marked MW. The MW column has a formula in it to calculate the molecular weight of the protein based on the mm in the preceeding column. mm MW (Do not write anything in the MW column or your will erase the formulas.) 0.0 0.0 0.0 0.0 y = aX 2 + bX + c 10 20 30 40 50 1 10 100 1000 protein standards mm from origin molecular weight /1000 polynomial regression Page 2 T . .)...
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This note was uploaded on 03/28/2011 for the course BIO 311 taught by Professor Staff during the Fall '08 term at SUNY Stony Brook.

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2010 Bio 311 Lab E Master Standard - and the "c"...

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