Lecture 6 - BIO 311 pET and DE3 Explained Escherichia coli...

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BIO 311 pET and DE3 Explained
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Escherichia coli Gram-negative rods Prescott Microbiology, 2002 Molecular Biology Factory 3200 genes in one chromosome
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The bacterial cell has one large chromosome Cell Wall Cell Wall The bacterium becomes permanently “transformed” if the The bacterium becomes permanently “transformed” if the plasmid DNA integrates into the chromosomal DNA plasmid DNA integrates into the chromosomal DNA X DE3
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DE3 Bacteria and the pET System 1. In DE3 bacteria, the gene for T7 RNA polymerase has been place in the E. coli chromosome under control of the lac operon 1. Expression of the T7 RNA polymerase is induced through the lac operon using IPTG DE3 bacteria have been permanently modified
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Classic system of Lac Operon in E. coli Lac operon made of several elements and codes for three proteins 1. lac Z => beta-galactosidase = breaks lactose --> glucose + galactose 2. lac Y => lac permease= lactose transportation into cell 3. lac A => acetyltransferase
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This note was uploaded on 03/28/2011 for the course BIO 311 taught by Professor Staff during the Fall '08 term at SUNY Stony Brook.

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Lecture 6 - BIO 311 pET and DE3 Explained Escherichia coli...

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