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2010 Bio 311 Final Exam Sample Questions 2 Key

# 2010 Bio 311 Final Exam Sample Questions 2 Key - BIO 311...

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BIO 311 Fall 2010 Dr. A.K.M. Mollah Choose the correct answer: A. 1. You wish to make 100 mL of 1X TBS-T solution (1X TBS with 0.1% weight/vol TWEEN-20, a detergent). Your lab however, only has 10X TBS and 5% TWEEN-20 solutions, therefore you would combine: a) 10 mL of 10X TBS with 2 mL of 5% TWEEN-20 and 88 mL of distilled water. b) 1 mL of 10X TBS with 5 mL of 5% TWEEN-20 and 94 mL of distilled water. c) 10 mL of 10X TBS with 20 mL of 5% TWEEN-20 and 70 mL of distilled water. d) 3 mL of 10X TBS with 2 mL of 5% TWEEN-20 and 95 mL distilled water. e) 40 mL of 10X TBS with 50 mL of 5% TWEEN-20 and 10 mL distilled water. A. 2. After thawing frozen solutions it is wise to first “vortex” the solution and then centrifuge it briefly. The reason for this is: a) Many substances tend to form gradients when frozen, so vortexing them assures they are mixed evenly, the subsequent centrifugation simply pulls the liquid to the bottom of the container to make working with it more convenient. b) Frozen substances tend to be well mixed, and the vortexing is simply the product of a nervous mind. c) Vortexing functions to bring all of the liquid to the bottom while centrifugation mixes

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2010 Bio 311 Final Exam Sample Questions 2 Key - BIO 311...

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