lectures 20-22 2011

lectures 20-22 2011 - Testosterone Positive Sexual behavior...

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3/29/11 Testosterone Sexual behavior Aggressive behavior (reproduction) Sperm production Negative feedback High energy costs Reduced fat stores Increased mortality Increased injury Interferes with Positive Negative
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3/29/11
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3/29/11 Semen Analysis Animal agriculture versus human medicine 1.Visual inspection -color -volume -viscosity 2. Microscope inspection -Abnormal sperm (bent bodies, two heads, etc.) -motility (general motion, forward is best) -spermacytometer- count sperm cells, calculate concentration -live/dead stain- composed of cosin and negrosin, will not penetrate plasma membrane of live sperm 3. Ethidium bromide exclusion test -measured with a fluorometer -will not penetrate plasma membrane of live cell -RNA/DNA stained by ethidium bromide 4. Spermatocrit -packed cell volume to measure concentration 5. Photometric Methods *cost issue for farm use
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3/29/11
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3/29/11
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3/29/11
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3/29/11 Inhibin and Males Blood Inhibin-B Exceptions rams and roosters Inhibin-A – not detected
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3/29/11
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3/29/11 Expression of inhibin and activin Inhibin Sertoli cells source of endocrine Leydig capable Spermatocytes? Activin
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lectures 20-22 2011 - Testosterone Positive Sexual behavior...

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