Ch19-TCF

Ch19-TCF - Quantitative Chemical Analysis Quantitative...

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Quantitative Chemical Analysis Quantitative Chemical Analysis Chapter 19: Applications of Spectroscopy Applications of Spectroscopy
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2 Dealing with mixtures of analytes (IV) (V)
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3 Beer-Lambert Law
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3a Beer-Lambert Law
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4 Can use Solver or Linest This is a Solver file: You will use Linest in your laboratory to quantitate three components of an over-the-counter analgesic tablet using UV spectroscopy. A sample Excel file will be emailed to you.
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4a y = m 1 x 1 +m 2 x 2 + m 3 x 3 + b Total m 1 m 2 m 3 Observed Wave- Specific Absorptivities Absorbance length Aspirin Acetamin Caffeine of Unknown 190 0.2094 0.1777 0.0565 1.345 195 0.1910 0.1470 0.0701 1.182 200 0.1392 0.1579 0.1066 1.150 205 0.0791 0.0983 0.1335 0.763 245 0.0007 0.0623 0.0120 0.286 275 0.0002 0.0131 0.0487 0.113 Linest Answers m 3 m 2 m 1 b Const = true Caffeine Acetamin Aspirin constant slope, m 1.084 4.403 2.408 -0.001 std dev m 0.057 0.110 0.075 0.006 std dev b R sqrd 0.99996 0.005 #N/A #N/A SE(y) F statistic 16300 2 #N/A #N/A dof Const = false slope, m 1.0795 4.3888 2.4158 0.0000 b set to 0 std dev m 0.0434 0.0672 0.0519 #N/A R sqrd 0.99999 0.0043 #N/A #N/A SE(y) F statistic 95848 3 #N/A #N/A dof
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5 Isosbestic points are useful in assessing purity of a mixture of two components where one is being converted into the other.
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6 Use of fluorescent labels
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This note was uploaded on 03/28/2011 for the course CHEM 300L at USC.

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Ch19-TCF - Quantitative Chemical Analysis Quantitative...

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