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Unformatted text preview: (epl gal ern) opr rds esw nal rpc gly dlg Draw a map of the relative positions of these markers and include the position and orientation of the F plasmid for each Hfr strain: ii. Arthur figured Muggles would have to figure out the order of the three genes that were too close to together to resolve, so to emulate them he mated HfrY to the F- strain for long enough for the epl marker to transfer. He then selected for Epl + recombinants and counted how many of those were also Gal + and Ern + : # recombinants Epl + Gal + Ern + 620 + + + 114 + +-35 +-+ 2 +--Draw a map of the epl , gal , and ern markers with distances between them: iii. Arthur realizes that to really study the epl , gal , and ern markers he would need an F’ plasmid with all three of them on it. Which of the preceding Hfr strains should he use to isolate such an F’ plasmid?...
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This note was uploaded on 03/28/2011 for the course LS 4 taught by Professor Ribaya during the Spring '08 term at UCLA.
- Spring '08