wk5 - (epl gal ern) opr rds esw nal rpc gly dlg Draw a map...

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Covel Peer Learning – LS 4 (Rachel Care) Week 5 Attach the last 2 pages of last week’s handout to this. Voila – insta-handout! d. Fun with Hfr strains As part of his continuing Muggle Studies, Arthur Weasley has been trying his hand at molecular biology. Starting with an F+ strain that was prototrophic and Fer S , he isolated several Hfr strains. He mated these different Hfr strains to the same F- strain: F-: Fer R , Rds - , Ern - , Dlg - , Nal - , Gly - , Esw - , Opr - , Rpc - , Gal - , Epl - i. Interrupted mating experiments showed Mr. Weasley that the Hfr strains transferred the wild-type alleles in the order listed below as the mating was allowed more and more time. Hfr strains Order of transfer (first to last ) HfrP dlg gly rpc nal esw rds opr (epl gal ern) HfrE dlg (epl gal ern) opr rds esw nal rpc gly HfrR rpc nal esw rds opr (epl gal ern) dlg gly HfrC rds esw nal rpc gly dlg (epl gal ern) opr HfrY
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Unformatted text preview: (epl gal ern) opr rds esw nal rpc gly dlg Draw a map of the relative positions of these markers and include the position and orientation of the F plasmid for each Hfr strain: ii. Arthur figured Muggles would have to figure out the order of the three genes that were too close to together to resolve, so to emulate them he mated HfrY to the F- strain for long enough for the epl marker to transfer. He then selected for Epl + recombinants and counted how many of those were also Gal + and Ern + : # recombinants Epl + Gal + Ern + 620 + + + 114 + +-35 +-+ 2 +--Draw a map of the epl , gal , and ern markers with distances between them: iii. Arthur realizes that to really study the epl , gal , and ern markers he would need an F’ plasmid with all three of them on it. Which of the preceding Hfr strains should he use to isolate such an F’ plasmid?...
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This note was uploaded on 03/28/2011 for the course LS 4 taught by Professor Ribaya during the Spring '08 term at UCLA.

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wk5 - (epl gal ern) opr rds esw nal rpc gly dlg Draw a map...

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