LS4_12_phagegenetics

LS4_12_phagegenetics - Phage Genetics Mutations in...

Info iconThis preview shows pages 1–5. Sign up to view the full content.

View Full Document Right Arrow Icon
Phage Genetics: Mutations in bacteriophage
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
• Spread E. coli cells on agar growth medium in a sterile petrie dish and grow at 37º C overnight. Phage Plaques -Eventually all of the bacteria in the vicinity of the original phage particle will be lysed yielding a clear spot (hole) in the lawn of bacteria, called a plaque. -Each overnight plaque contains ~1 x 10 8 bacteriophage. -Formation of “lawn” of bacteria on the surface of the medium. • If you add a single bacteriophage (T4 or T2), it will infect one bacterium, lyse it, and release 300 progeny phage about 25 minutes later…infecting neighboring bacteria (cycle repeats)
Background image of page 2
• Most phage phenotypes are visualized as plaques on lawns of bacteria • Plaques can vary in morphology: - Large or small plaques are determined by how fast lysis occurs - Host range is a reflection of what strains of bacteria the phage can bind to and lyse Observing Phage
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
• Wild type T4 phage produce small plaques with fuzzy margins, while rapid lysis (r) mutants produce large plaques with sharp margins.
Background image of page 4
Image of page 5
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

Page1 / 16

LS4_12_phagegenetics - Phage Genetics Mutations in...

This preview shows document pages 1 - 5. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online