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Unformatted text preview: Covel Peer Learning LS 4 (Rachel Care) Week 8 Week 7 Material: 1. Suppressors of nonsense mutations a. Open reading frames: How many possible ORFs extend through the sequence below? 5 CTTACAGTTTATTGATACGGAGAAGG 3 3 GAATGTCAAATAACTATGCCTCTTCC 5 b. The nonsense suppressor is a ______ gene with a mutated ____________, which recognizes the nonsense _______ __________ and places an _________ _______ there instead. c. i. A protein in E. coli is normally encoded by the following sequence: 5-AUGGUGUACGACAAGAGAUAA-3 What is the encoded amino acid sequence? (see table on last page) ii. In a certain strain of E. coli, a mutation causes the following change: 5-AUGGUGUA G GACAAGAGAUAA-3 What is the amino acid sequence in this mutant strain? iii. Later, the mutation in (ii) above is found to revert. Sequencing shows that the DNA is still in the sequence shown in (ii). How is the wild type phenotype occurring? (List all the possible ways and be clear about your directionality!) 2. lac operon basics (vocab!) operon:...
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This note was uploaded on 03/28/2011 for the course LS 4 taught by Professor Ribaya during the Spring '08 term at UCLA.
- Spring '08