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Unformatted text preview: Covel Peer Learning – LS 4 (Rachel Care) Week 8 Week 7/8 Material: 1. Suppressors of nonsense mutations a. Open reading frames: How many possible ORFs extend through the sequence below? XOO (UAG) 5’ …CTTACAGTTTAT TGA TACGGAGAAGG… 3’ 3’ …G AAT GTCA AAT AACTATGCCTCTTCC… 5’ (UGG) (UGG) OXX b. The nonsense suppressor is a _ tRNA _ gene with a mutated _ anticodon __, which recognizes the nonsense __ stop _ _ _codon __ and places an __ amino __ __ acid _ there instead. c. i. A protein in E. coli is normally encoded by the following sequence: 5’-AUGGUGUGCGACAAGAGAUAA-3’ What is the encoded amino acid sequence? Met-Val-Cys-Asp-Lys-Arg-STOP ii. In a certain strain of E. coli, a mutation causes the following change: 5’-AUGGUGUG A GACAAGAGAUAA-3’ What is the amino acid sequence in this mutant strain? Met-Val-STOP iii. Later, the mutation in (ii) above is found to revert. Sequencing shows that the DNA is still in the sequence shown in (ii). How is the wild type phenotype occurring? (List all the possible ways and be clear about your directionality!)occurring?...
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This note was uploaded on 03/28/2011 for the course LS 4 taught by Professor Ribaya during the Spring '08 term at UCLA.
- Spring '08