midtermIIreviewmans

midtermIIreviewmans - Covel Peer Learning LS 4(Rachel Care...

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Covel Peer Learning – LS 4 (Rachel Care) Review Session for Midterm II (McWhorter) – lectures 7-15 1. Recombination analysis: mapping deletion and point mutations Studying the rII locus, Professor Trelawny has divined that mutations 1 through 6 are deletions. The following table summarizes the results of crossing each of the mutations: 1 2 3 4 5 6 1 - + - + - 2 + - + + 3 + - - 4 + + 5 + 6 Map the deletions: (see below) For extra credit, Hermione has found 7 more mutations which are all point mutations. This table illustrates the results of crossing each point mutation to each deletion: 1 2 3 4 5 6 A + + + - + + B + + + + + - C + + - + - + D - - + - + + E + + + + + - F - + + + + + G - + + - + + Map the point mutations onto the deletion map: 2 5 4 3 1 6 A B B,E F D G A C b,e, and f complement rII B mutants but not rIIA mutants. They also complement deletion 2 but not deletion 1. Where is the rIIA / rIIB boundary? (draw it on your map)
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2. Another variant on your average complementation/recombination problem L, M, N, O, P and Q are strains of Neurospora auxotrophic for alanine. The heterokaryon strains resulting from mating haploids were plated on minimal media with the results tabulated below: L M N O P Q L + + + + + M - + + + N + + + O - + P + The heterokaryon were then allowed to sporulate and 1000 spores from each cross were germinated on minimal media. The number of colonies that grew are below: L M N O P Q L 350 350 200 200 50 M 0 150 150 400 N 150 150 400 O 0 250 P 250 How many genes are involved in the alanine biosynthetic pathway? 4 : M,N
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midtermIIreviewmans - Covel Peer Learning LS 4(Rachel Care...

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