Covel Peer Learning – LS 4 (Rachel Care)
Week 6
Week 5/6 Material:
1. Transduction and Bacteriophage vocab: Week 5
a.
How do we observe bacteriophage?
b.
Selection vs. screen:
c. What are the two classes of phage life cycles?
1.
2.
i. Describe the 2 stages of the life cycle:
ii. Define “prophage”:
iii. Define “repressor”:
iv. Define “zygotic induction”:
d. What are the two types of transduction?
1.
2.
i. What are the two main differences between them?
1.
2.
ii. Define “transductant”:
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iii. Define “co-transduction”:
map out a co-transduction problem:
goal:
formula for co-transduction frequency:
steps:
1.
2.
3.
4.
2. Vocab: Week 6 (yes theres more, yes its important)
central dogma:
rII:
permissive:
non-permissive:
high m.o.i.:
deletions:
point mutations:
complementation analysis:
recombination analysis:
formula for recombination frequency:
fine structure map:
3. Transductant genotypes and co-transduction frequencies
Donor strain genotype is: Str
+
trp
+
ala
+
Recipient strain genotype is: Str
-
trp
-
ala
-
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- Spring '08
- Ribaya
- DNA, recessive mutations, rapid lysis, co-transduction frequency, independent recessive mutations
-
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