wk7 - B / K at high / low m.o.i. and plate progeny phage on...

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Covel Peer Learning – LS 4 (Rachel Care) Week 7 Week 6/7 Material: 1. Important review from last week! a. Wildly Important Table (also known as “that slide Laski loves”) RECOMBINATION COMPLEMENTATION Tests for: Infect: Why: How does this work? Plate: Why: “+” means: “-“ means: if “+”, expect: B < > = K B < > = K if “-”, expect: B < > = K B < > = K b. Ok, now let’s practice! To figure out if two different rII point mutations are in the same cistron, infect E. coli B / K at high / low m.o.i. and plate progeny phage on E. coli B / K . If plaques do / don’t appear on B / K and / not B / K , the mutations are in the same / different cistron. To figure out if two different rII point mutations are in the same nucleotide, infect E. coli
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Unformatted text preview: B / K at high / low m.o.i. and plate progeny phage on E. coli B / K . If plaques do / dont appear on B / K and / not B / K , the mutations are in the same / different cistron. 2. Genetic evidence for the triplet code: rII- frameshift mutations (Crick) Step 1 What: How: Why: Step 2 What: How: Why: Step 3 What: How: Why: Step 4 What: Revertant: Suppressor: How: 1. Illustration: 2. Illustration: Step 5 What: How: Why: Step 6 What: How: Why: If cross gives rII+ recombinant progeny, mutation is: If cross gives rII- recombinant progeny, mutation is: Step 7 What: How: Check: If rII+ results If rII- results Why: Step 8 What: How: Why:...
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wk7 - B / K at high / low m.o.i. and plate progeny phage on...

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