CHAPTER-20 - CHAPTER 20 DNA Technology and Genomics Chapter...

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CHAPTER 20 DNA Technology and Genomics Chapter Objectives DNA Cloning 1. Explain how advances in recombinant DNA technology have helped scientists study the eukaryotic genome. 2. Describe the natural function of restriction enzymes and explain how they are used in recombinant DNA technology. 3. Explain how the creation of sticky ends by restriction enzymes is useful in producing a recombinant DNA molecule. 4. Outline the procedures for cloning a eukaryotic gene in a bacterial plasmid. 5. Describe techniques that allow identification of recombinant cells that have taken up a gene of interest. 6. Define and distinguish between genomic libraries using plasmids, phages, and cDNA. 7. Describe the role of an expression vector. 8. Describe two advantages of using yeast cells instead of bacteria as hosts for cloning or expressing eukaryotic genes. 9. Describe two techniques to introduce recombinant DNA into eukaryotic cells.
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