2010_midterm_exam_2 - Biol 241, Spring 2010 Midterm Exam II...

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Biol 241, Spring 2010 Midterm Exam II MC questions (100 points, 2.5 points each question) 1) Under which of the following conditions would a lac operon produce the greatest amount of β –galactosidase? a) Lactose present in the media and the intracellular cAMP level is low. b) Lactose present in the media and the intracellular cAMP level is high. c) Lactose absent in the media and the intracellular cAMP level is low. d) Lactose absent in the media and the intracellular cAMP level is high. 2) Which of the following events is usually associated with an increase in gene expression in eukaryotes? a) The deacetylation of histone tails by histone deacetylation complex. b) The rearrangement histones by SWI/SNF chromatin remodeling complex. c) The binding of factors to silencers. d) The methylation of DNA by methyl transferases. 3) Loss-of-function mutations in E. coli dam methylase are likely to: a) Increase the mutation rate because mismatch system cannot distinguish the newly synthesized strand. b) Increase the mutation rate because less time for mismatch to correct the mistakes. c) Increase the mutation rate because the DNA is less sensitive to digest by restriction enzymes. d) Increase the mutation rate because methylated DNA has unusual base- pairing properties. 4) Which of the following is an example of akylating agent? a) Acridine orange. b) Ethylmethane sulfonate. c) 5-bromouracil. d) 2-aminopurine. 5) Which of the following systems is an example of error-prone repair? a) Mismatch repair. b) Nucleotide excision repair. c) Photoreactivation repair. d) SOS repair.
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6) Which one of the following enzymes has the ability to synthesize RNA using DNA template? a) DNA polymerase I. b) DNA polymerase III. c) Telomerase. d) Primase . 7) Which one of the following enzymes has the 3’-5’ exonuclease activity? a) Telomerase. b) RNA polymerase. c) DNA polymerase III. d) Gyrase. 8) Which one of the following experiments could demonstrate that eukaryotic DNA is complexed with histones? a) Ames test. b) Fluctuation test. c) Nuclease hypersensitive test. d) Hershey-Chase blender experiment. 9) A 200 bps circular DNA has 20 helical turns, this DNA is likely to assume the conformation of: a) Relaxed circle. b) Positive supercoil. c) Negative supercoil. d) Lampbrush chromosome. 10) The replication of yeast chromosome is initiated from: a) Pribnow box. b) Ori. c) Autonomous replicating sequence. d) Hogness sequence.
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This note was uploaded on 03/30/2011 for the course BIOL 241 taught by Professor Henrychang during the Spring '10 term at Purdue University-West Lafayette.

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2010_midterm_exam_2 - Biol 241, Spring 2010 Midterm Exam II...

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