Problem_set_2_key

Problem_set_2_key - BIS104-002 Winter 2010 Problem Set II...

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BIS104-002 Winter 2010 Problem Set II “Due” Jan 29. This problem set will not be collected, but you should do it before looking at answers. Answers will be posted on Jan 29. 1-3. Revisit the paper you read for Problem Set #1, “Global analysis of protein localization in budding yeast” Nature. 2003 Oct 16;425:686-91. A PDF version of the paper is available on the class website. Application: Go the web site that provides images of every GFP fusion from the above study (http://yeastgfp.yeastgenome.org/). Feel free to explore the site as you answer the following questions: 1. On Problem set #1 you were asked where Sec 61 and Sec 33 localize. Compare the localization patterns of Sec61 and Sec33. How do these patterns differ? The GFP fusion with Sec61 localizes around the nucleus in a pattern that is consistent with the endoplasmic reticulum. In contrast, Sec33 localizes in the golgi compartment. 2. Although they are both involved in secretion, do their localization patterns suggest that they have different roles? Why? What is the biochemical function of Sec61 and Sec33 (click on the blue YLR or YDL name on the yeastgfp entry and read the description on the SGD page)? These patterns suggest that sec61 is involved in an early step in secretion (i.e., ER) and Sec33 has a role later in the secretory pathway (i.e., golgi). Consistent with
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This note was uploaded on 03/30/2011 for the course BIS 104 taught by Professor Scholey during the Spring '08 term at UC Davis.

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Problem_set_2_key - BIS104-002 Winter 2010 Problem Set II...

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