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Unformatted text preview: BIS104 Winter 2010 Problem Set 4 “Due” March 1. This problem set will not be collected, but you should do it before looking at answers. Answers will be posted on March 1. 1-4. You have microinjected a newt lung cell with rhodamine-labeled tubulin, caged- fluorescein labeled tubulin and the fluorescent DNA stain, DAPI. You wait until the cell enters metaphase and you uncage a bar of caged-fluorescein tubulin in the spindle. The figures below show the fluorecence intensity of uncaged fluorescein on the y axis and the distance from the left spindle pole (centrosome) on the x axis. The position of one sister chromatid that is attached to the left pole by a kinetochore fiber is also shown. 1. What is the rate of poleward flux in ¡ m min-1 ? 0.5 μ m/min 2. How will the rate of poleward flux be affected by the addition of taxol? Taxol will stabilze GDP-tubulin subunits and inhibit depolymerization. Since poleward flux requires loss of tubulin subunits at the minus end of the microtubule, poleward flux will be inhibited by taxol. 3. What will happen to the distance between the centrosome and the chromosome if you wait for 1 hour in the presence of taxol?...
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This note was uploaded on 03/30/2011 for the course BIS 104 taught by Professor Scholey during the Spring '08 term at UC Davis.
- Spring '08