Genome 371 Lecture 19 - Genome 371 Lecture 19 1/26/11 4:18...

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1/26/11 4:18 PM Genome 371 Lecture 19 Page 1 of 9 http://courses.washington.edu/au371mkr/resources/lecture_notes/lecture_19.html Lecture 19 Genetic analysis--recap 10 Dec 2010 Lecture 19 handout (pdf) Subscribe to podcast: Slide 1 Slide 2 Home Course mechanics Help hours Calendar Syllabus Lectures, Podcasts Quiz Sections Practice problems Exams GoPost Send email to class Useful links The Gradiator
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1/26/11 4:18 PM Genome 371 Lecture 19 Page 2 of 9 http://courses.washington.edu/au371mkr/resources/lecture_notes/lecture_19.html Slide 3 Slide 4 A question that came up frequently in quiz section was, How did they know to do the LOD score analysis for chromosome 6? The answer is, they didn't know. They picked a minimal set of polymorphic sites distributed throughout the genome and did the LOD score analysis on the full set, generating a large table consisting of LOD values for sites across the whole genome. What they reported then in this figure is just that portion of the genome where they got "interesting" scores. Slide 5 Back then, to test genotypes at microsatellite markers, PCR reactions and gels like the example you saw had to be run for each marker to be tested, one at a time. These days, with the availability of "SNP chips" to do allele-specific oligo hybridization (and even more recently, with sequencing becoming cheaper) it is possible to test the genotypes at hundreds of thousands of polymorphic loci in a single experiment. Slide 6
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Genome 371 Lecture 19 - Genome 371 Lecture 19 1/26/11 4:18...

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