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Gen371 Practice 5 Answers - Gen371 Practice 5 Answers 4:19...

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1/26/11 4:19 PM Gen371 Practice 5 Answers Page 1 of 10 http://courses.washington.edu/au371mkr/resources/problems/problems_05-key.html Practice problems set 5 -- answers 1. One of the transposon insertions isolated from a Drosophila mutagenesis experiment is shown; restriction enzyme sites (BamHI, ClaI, MscI) and potential primers ( , , etc.) are marked. If you wanted to clone some portion of the interrupted fly DNA without adding a vector, which restriction enzyme(s) could you use? (Assume that you would do a complete digest.) Enzyme Use? Not use? Why? BamHI Don't use: Amp R would be separated from ori, therefore no fragment would give stable E. coli transformants. ClaI Use: ori and Amp R stay together, some portion of fly DNA (to the right) would be included in the circularized molecule. MscI Don't use: the ori+Amp R fragment would contain none of the flanking fly DNA and therefore wouldn't be useful. Which primer(s) would be best for sequencing the cloned fly DNA? 4 and 5 (one at a time!) Suggestion for further practice (may have come up in quiz section): In this question, you are asked to clone the flanking fly DNA as a way of amplifying that DNA prior to DNA sequencing. Starting with the same mutant fly, can you come up with a way of amplifying that same fly DNA using PCR instead of cloning in E. coli? 2. The pedigree shows inheritance of two linked autosomal recessive traits, alkaptonuria and disaccharide intolerance. (a) In the boxes below individuals II-1, II-2, III-1, and III-2, write out their genotypes showing the linkage with the alleles in the correct phase. Home Course mechanics Help hours Calendar Syllabus Lectures, Podcasts Quiz Sections Practice problems Exams GoPost Send email to class Useful links The Gradiator
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1/26/11 4:19 PM Gen371 Practice 5 Answers Page 2 of 10 http://courses.washington.edu/au371mkr/resources/problems/problems_05-key.html (b) Which grandchild (III-1 or III-2) shows clear evidence of having received a recombinant gamete? What was the genotype of that recombinant gamete? Grandchild with recombinant gamete: III-2 Genotype of that recombinant gamete: A D Suggestion for further practice: This is a very simple extension, but worth doing just to get yourself comfortable with the process... In this question, you are just told that the two loci are linked. To extend this question, make up a map distance and then predict the probability of child III-1's genotype vs. child III-2's genotype from the mating that is given. 3. A plant species produces interesting flowers in which wild type flowers have two white petals and two colored (pigmented) petals. It is known that wild type flower coloring requires at least two genes, A and B. There are two competing ideas to explain the flower color pattern: Idea 1: Pigment is produced by an enzyme encoded by wild type Gene B, but transcription of Gene B requires the protein made by Gene A.
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