Gen371 Practice 6 Answers

Gen371 Practice 6 Answers - Gen371 Practice 6 Answers 4:19...

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1/26/11 4:19 PM Gen371 Practice 6 Answers Page 1 of 17 http://courses.washington.edu/au371mkr/resources/problems/problems_06-key.html Practice problems set 6 -- answers 1. The population of wild cheetahs is diminishing rapidly. Captive breeding programs at zoos are struggling due to the sterility of some mating pairs. In each case of sterility, the male produces no motile sperm. The zoo veterinarian suspects that one of three LINKED genes may be to blame. She develops PCR assays for the polymorphic markers A, B, and C (microsatellite loci) that are tightly linked to each gene. She uses these assays to analyze DNA from male and female cheetahs at the San Diego, Washington DC, and St. Louis zoos. A gel with her results is shown to the right. (a) Why do the males have three bands? These three linked loci are on the X chromosome. Males have only one X and no alleles on the Y. (b) Why do the females have five bands? Although females have two X chromosomes and could have as many as 6 bands, each is homozygous for one of the markers, resulting in only five bands. (c) Based on these data, which allele is associated with the sterility? B1 Over the years, the Washington DC pair produces many offspring. PCR analysis of the DNA from 8 sons reveals the inheritance pattern shown to the right. (d) In the mother, which A, B and C alleles are present on each homologue? A1, B2, C1 and A2, B1, C1 (e) Which son(s) carries a recombinant chromosome? Home Course mechanics Help hours Calendar Syllabus Lectures, Podcasts Quiz Sections Practice problems Exams GoPost Send email to class Useful links The Gradiator
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1/26/11 4:19 PM Gen371 Practice 6 Answers Page 2 of 17 http://courses.washington.edu/au371mkr/resources/problems/problems_06-key.html Son #4 (A1, B1, C1) (f) Son #7 is unusual. Which chromosomes and what alleles did he receive from Mom? One X with the A2, B1, and C1 alleles (g) How did son number 7 arise? Be specific. Non-disjunction in meiosis I in the father. 2. PCR amplification was done simultaneously on multiple polymorphic microsatellite repeat (VNTR) loci on DNA from a family of eleven--mom, dad, eight biological children, and one adopted child. The combined PCR results for each person are shown on the gel diagram. The PCR products in Mom's samples are labeled A-F. To label any of the other PCR products, use the space between lanes. The horizontal dashed lines are to help you see the alignment of bands across the gel. Mom's sample is shown three times for convenience. (a) Which lane corresponds to Dad's DNA sample? Mark the PCR fragments in Dad's lane as G-L (top to bottom) in the figure above. Dad's sample is: 2 Explanation: No alleles shared with Mom, but 3 alleles shared with each of the others (except one, who would be the adopted child). (b) How can you tell from the PCR results that neither parent is homozygous for any of the loci being tested?
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This note was uploaded on 03/31/2011 for the course GENOME 371 taught by Professor Unsure during the Spring '03 term at University of Washington.

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Gen371 Practice 6 Answers - Gen371 Practice 6 Answers 4:19...

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