Lab 6b ak - Akshay Patel Period 1 Lab 6B The Effect of Gel Electrophoresis on DNA Fragments Abstract The objective of this experiment is to see the

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Akshay Patel 4/2/11 Period 1 Lab 6B: The Effect of Gel Electrophoresis on DNA Fragments Abstract: The objective of this experiment is to see the effect of electrophoresis on DNA cleaved by restriction enzyme. From the information known, it can be inferred that if a sample of DNA is cut by EcoRI or HindIII and electrophoresis occurs, then the DNA fragments will migrate toward the positive electrode with the bands moving to different locations based on their size. In this experiment, DNA fragments are going to be cut by restriction enzymes and separated by gel electrophoresis. The results from this experiment support the hypothesis since the DNA fragments moved towards the positive charge and the smaller fragments moved faster than the larger fragments. Problem: What is the effect of gel electrophoresis on DNA that is cleaved by restriction enzymes? Introduction: DNA is cleaved by a restriction enzyme so the DNA fragments can be created and separated through gel electrophoresis. Restriction enzymes are used to cut up DNA. Restriction enzymes cut DNA at specific recognition sequences of nucleotides. Restriction enzymes make a cut across a double-stranded DNA that is staggered. This produces fragments that have one strand of DNA that extends beyond the complementary strand that is the sticky end. In this process, DNA fragments of different lengths are separated as they move through the gel under the influence of the electric field in the apparatus. Since DNA is negatively charged due to its phosphate groups, it will move towards the positive electrode. The shorter fragments will move farther than the larger fragments. In this experiment, three samples of DNA from bacteriophage Lamba will be cleaved by EcoRI restriction enzyme and HindIII restriction
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This note was uploaded on 04/02/2011 for the course BIOLOGY 101 taught by Professor Martin during the Spring '08 term at Rutgers.

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Lab 6b ak - Akshay Patel Period 1 Lab 6B The Effect of Gel Electrophoresis on DNA Fragments Abstract The objective of this experiment is to see the

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