ER, Protein Folding, Glyco(5)

ER, Protein Folding, Glyco(5) - ER Protein Folding and...

Info iconThis preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon
Click to edit Master subtitle style ER Protein Folding and Modifications Folding pathways
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Figure 6-85 Molecular Biology of the Cell Protein Folding Pathways Figure 6-83 Molecular Biology of the Cell Molten globule Correctly Folded Proteins acquire their 3-dimensional structure through folding intermediates Some molten globules can be trapped in “ off-pathway ” structures Molten globules to aggregates
Background image of page 2
Formation of Disordered Protein Aggregates U nfolded N ative I ntermediate Hsp70 organization Figure 6-86 Molecular Biology of the Cell (© Garland Science 2008) Hsp70 System
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Binding Protein (BiP): a Molecular Has 2 domains ATPase domain N-terminal, 450 amino acids Peptide binding domain C-terminal 200 residues Functions of BiP ER -resident protein; hsp70 family member Prevents aggregation of incorrectly folded proteins Disaggregation of proteins (also reported) Binds to hydrophobic patches on proteins Patches are normally found in interior of proteins Hsp70 binding/release
Background image of page 4
Click to edit Master subtitle style Hsp70: Peptide Binding/Release Cycle Hsp70•ADP binds tightly to peptide Peptide release is coupled to ADP exchange for ATP J-domains • Hsp70 is a weak ATPase • Peptide raises ATP hydrolysis 500% New Life!
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 6
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/03/2011 for the course CBIO 3400 taught by Professor Shen,kipreos during the Spring '08 term at UGA.

Page1 / 20

ER, Protein Folding, Glyco(5) - ER Protein Folding and...

This preview shows document pages 1 - 6. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online