Signal_Peptide_hypothesis(3)0

Signal_Peptide_hypothesis(3)0 - Organelle Isolation: Signal...

Info iconThis preview shows pages 1–6. Sign up to view the full content.

View Full Document Right Arrow Icon
Click to edit Master subtitle style Organelle Isolation: Signal Peptide Hypothesis Organelles, proteins and lipids
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Cells Contain Several Organelles • Organelles have different lipid and protein compositions • Most protein synthesis takes place in the cytosol How do organelles acquire different lipid and protein content? Techniques: Protein targeting to organelles
Background image of page 2
Microscopy Discussed : see ER protein in previous lecture Organelle Purification and Study In Vitro • Organelles can be purified from cells Organelles can be active in vitro Strategy • Homogenize cells (or tissue): ER fragments into microsomes • Separate organelles by sedimentation Velocity sedimentation (centrifugation) Equilibrium sedimentation • Cell-free system developed How to Analyze Targeting of Proteins to Organelles Velocity sedimentation
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Organelles can be separated using Sedimentation velocity properties and by their density Velocity Sedimentation Equilibrium sedimentation Figure 8-9 Molecular Biology of the Cell Sequential sedimentation at different speeds
Background image of page 4
Properties of Organelles: Sedimentation and Density Figure 5-24 Purity of Isolated Organelles Microscopy
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 6
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/03/2011 for the course CBIO 3400 taught by Professor Shen,kipreos during the Spring '08 term at University of Georgia Athens.

Page1 / 20

Signal_Peptide_hypothesis(3)0 - Organelle Isolation: Signal...

This preview shows document pages 1 - 6. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online