Howlett-Supplemental

Howlett-Supplemental - Supplementary Material PCR...

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Supplementary Material PCR amplification of the BRCA2 gene The components of the PCR reaction were: 20mM Tris-HCl(pH8.4), 50mM KCl, 1.5mM MgCl 2 , 0.1mM in each of dATP, dCTP, dGTP, TTP, 0.1μM of each primer, 5ng/μl DNA, 0.05units/μl Taq polymerase (Taq Platinum, GIBCO BRL, Gaithersburg, MD). The primer sequences are as follows: Exon Primer name Sequence Amplicon size 2 2-F GTT CCA GGA GAT GGG ACT GAA 348 bp 2-R ACA CAT AAG GAA CAG TTT ATG GTT 3 3-F CCA TAG TCA AGA TCT TTA GCA 466 bp 3-R ACT GAT TTG CCC AGC ATG ACA 4 4-F TTA CAA CTC CCT ATA CAT TCT CA 454 bp 4-R AAC CAG CCA ATT CAA CAT CAC A 5/6 5/6-F ATA TCT AAA AGT AGT ATT CCA ACA A 421 bp 5/6-R AAT TGC CTG TAT GAG GCA GAA T 7 7-F GTT ATA CCT TTG CCC TGA GAT T 398 bp 7-R GTC AGT TAC TAA CAC ACT TAT CA 8 8-F GTT TAT TCA CTG TGT TGA TTG AC 372 bp 8-R CAT ATA GGA CCA GGT TTA GAG A 9 9-F CAT CAC ACT ACT CAG GAT GAC A 495 bp 9-R GCA TGG TGG TGC ATG CTT GTA 10a 10a-F CCA AGT ACT CAG AAT AAC CCT T 497 bp 10a-R TTT GTC ACT TCC ACT CTC AAA G 10b 10b-F TCC ATG AAG CAA ACG CTG ATG A 470 bp 10b-R CCA GAT ATT GCC TGC TTT ACT G 10c 10c-F GAC CTA TTA GAC ACA GAG AAC A 454 bp 10c-R CTG CAT TCT TCA AAG CTA CAG A 10d 10d-F TCA GGT CAT ATG ACT GAT CCA A 416 bp 10d-R AAC ACA GAA GGA ATC GTC ATC T 10e 10e-F CCG AAA GAC CAA AAA TCA GAA CT 400 bp 10e-R AGC AAA CCA ACA TGG CAT ACG T 11a 11a-F CCA AAC ACT ACC TTT TTA ACT TAG T 380 bp 11a-R GAC CTC TTC TTT TAT ATC TGA AAC T 11b 11b-F CTG AAG AAC CAA CTT TGT CCT TA 350 bp 11b-R AGT GCT GGC ATT TTC ATG ATC AT 11c 11c-F TAT TAC CCC AGA AGC TGA TTC T 299 bp 11c-R TAC CTT TGA GCT TGT CTG ACA T 11d 11d-F ATG TCA CCC AGT ACA ACA TTC A 484 bp 11d-R CCT TTC ATT AGC AAC TTG GAA GA 11e 11e-F GAG AGT AGC ATC ACC TTC AAG A 464 bp
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11e-R CTG CCC ATT TGT TCA TGT AAT C 11f 11f-F AAA CAA GCA ACC CAA GTG TCA A 433 bp 11f-R CAG AAA CAA CTA CAC TAC TCT GT 11g 11g-F GGA AAT CAA GCT CTC TGA ACA T 403 bp 11g-R CAT CTG GTT TTC AGG CAC TTC A 11h 11h-F CCC CTC AGA TGT TAT TTT CCA A 387 bp 11h-R ACC CCA CTT CAT TTT CAT CTG T 11i 11i-F GGA ATG CAG AGA TGC TGA TCT 346 bp 11i-R CAT TGA AAC GAC AGA ATC ATG AC 11j 11j-F CTG CTC ATG GCA CAA AAC TGA 331 bp 11j-R GAA TTT CTA CTG GCA GCA GTA T 11k 11k-F CTT CTG CAG AGG TAC ATC CAA 496 bp 11k-R TGC TCC GTT TTA GTA GCA GTT A 11l 11l-F CTG ATC AGC ACA ACA TAT GTC T 470 bp 11l-R CTT TTC ATC ACG TTC GGG TTG T 11m 11m-F GAT CAG AAA CCA GAA GAA TTG C 423 bp 11m-R ACA CTT TGG GGC AGC TGT GAT 11n 11n-F TGC AAA GGA ATC TTT GGA CAA AG 408 bp 11n-R GCT AAG GCT GAA TTT TCA ATG AC 11o 11o-F GTG GTG CCA CCT AAG CTC TTA 386 bp 11o-R GTA TCT TGT TTT TCG GAG AGA TG 11p 11p-F ACT TCT GTG AGT CAG ACT TCA T 371 bp 11p-R TGT GGG TAT GCA TTT GCA TCT T 11q 11q-F CAG TAG CAT GTC TAA CAG CTA T 348 bp 11q-R GTT TCA TGT GAA ACA CAA ACG AT 11r 11r-F GAT ATT TGC GTT GAG GAA CTT G 328 bp 11r-R GCG TGC TAC ATT CAT CAT TAT C 11s 11s-F GGA TAG CCA GTG GTA AAA TCG T 498 bp 11s-R AGA CTT GCT TGG TAC TAT CTT CT 11t 11t-F TCA CCT TGT GAT GTT AGT TTG GA 469 bp 11t-R CAT TTT GTC TAG ACG TAG GTG AA
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This note was uploaded on 04/04/2011 for the course GENETICS 385 taught by Professor Brennemanandgordon during the Spring '11 term at Rutgers.

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Howlett-Supplemental - Supplementary Material PCR...

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