Second Exam - Oct 08

Second Exam - Oct 08 - Biochemistry 694:301 I\ Second Exam,...

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Unformatted text preview: Biochemistry 694:301 I\ Second Exam, Dr. Deis Tue. Oct. 28, 2008 I This exam consists of two parts. Part I is multiple choice. Each of these 25 questions is worth two points. Answer the Part I questions on this sheet, below. Answer the Part 11 questions on the question pages. Please use BLOCK CAPITAL letters like this --- A, B, C, D, E. Not lowercase! 1. 10. D 18. 2. 11. £38 19. M J0—. 12- _D_ 20. _C_ L L 3. fl 4. L 13. fi 21. 5. M 14. _C___ 22. 6. 15. 23. 7. Q9— 16 24. ELL... 8. 17. i9, 25. ___Q_ _ 9. _C_ GRADE PartITotal ____ P311111: II-l i I did not cheat on this exam. 11-2 la 0 694:301 Second Exam, page 2 l 2.* Galactosemia is A. curable using large doses of galactose a mild indigestion that affects many people worldwide C life-threatening genetic disease D. none of the above The su represented at right is H dis-a“ fi-D-Glucose D. f3 -D Fructose “0 V0 . l3 -D-Mannose . i3 -D-Glucosamine H a -D-Galactose /E’ (E- ott H O-T/H o H NameSe C { rl H . . C O The keto isomer of D-Ga-laetose—ts C C -:._ S u H I A. Galacturonic acid D. Fructose :2 H 0 — C a B. Galactitol E. Ribulose " H 0 “C " H C. Sedoheptulose C H 0 .. C H - c “0 H There seems to be a logical reason for the fact that in membranes, naturally C {Jig H occurring double bonds are “cis” rather than “trans.” Why? A. Producing “cis” double bonds requires less energy ® “cis” double bonds have a “kinked” shape that enhances fluidity ff. “cis” double bonds bind more easily to “L” Amino Acids. )1 In fact there is no reason, it was a random accident of nature. Ceramide contains sphingosine plus what? A. a fatty acid B. fatty acid, phosphate, sugar C. 2 fatty acids, phosphate, choline In the derivation of the Michaelis—Menten Equation, which assumption allows us to ignore the constant k2? A. initial rate = 0 is great If a given substrate has a low Km with an enzyme we usually assume A. weak binding C. reaction is very fast @. strong binding D. "substrate" is an inhibitor D. only sugar E. fatty acid, Pi, choline D. steady state 13- [E] = 8 What are the dimensions (units) of the the turnover number R2 or km? A. seconds D. mol/liter B. literslmol sec E. liter/Incl C} llsec The methyl glycoside of fructofuranose would be what? A. acetal D. hemiketal B. hemiacetal E. ester C. ketal F. none of the above 694:301 Second Exam, page 3 10. 11. 12. 13. 14.* 15. 16. 17. Nerve gas agents block what? A. sodium channels B. potassium channels C. the acetylcholine receptor @ acetylcholinesterase E. P-type ATPases Which G protein subunit spends most of its time bound to GDP? alpha C. gamma . beta D. delta If the target substrate for Protein Kinase C is RRGSE, then which sequence would be found on the “chew toy” or repressor protein? A. ARGSE RRGAE B. RAGSE . RRGSA C. RRASE When it‘s active, Protein Kinase C is . phosphorylated B. membrane associated C. globular and free floating D. dephosphorylated E. missing a regulatory subunit The figure at right represents A. an archaeon B. a gram positive bacterium @ a gram negative bacterium D. a eukaryotic cell Which of the following is a P-type ATPase? A. multidrug resistance receptor a the Ca+2 pump . lactose permease D. cystic fibrosis transmembrane reg. E. none of the above F. all of the above Many animal venoms (like that of the Cone Snail) contain ion channel blockers. Why are these particularly toxic? A. they interfere with digestion in the stomach B. they mimic epinephrine in the blood 0. they disrupt neurotransmission @all of the above Certain enzymes, like those in the Glycogen Breakdown Cascade, are active only when phosphorylated. Suppose you wanted to produce a version of the enzyme that is constitutiver active, i.e. active all the time? You can alter the gene and replace a certain A.SwithK D.TwithR B.SwithT E.AwithB C.SwithE ®SwithA 6942301 Second Exam, page 4 18. 19. 20. 21. 22. 24. Nam Gluconeogenesis takes place during intense exercise - why would an organism synthesize Glucose exactly at the time it is vigorously breaking it down? . there is no reason K37 muscle glycolysis produces lactate which is the fuel for gluconeo . glycolysis is in the cytoplasm while gluconeo is in the mitochondria glycolysis is in the liver while gluconeo is in the muscle cells E. none of the above In Gluconeogenesis, Fructose-1,6-bisphosphatase is inhibited by A. ATP ®AMP and F-2,6-BP B. Citrate E. Alanine and H+ C. Acetyl CoA What enzyme of Gluconeogenesis is found in the ER Lumen of liver cells? A. Pyruvate Kinase Enolase B. PEPCK a. G6Pase Q Fru-BP-ase Which of the following statements is true? A. anabolic pathways are generally inhibited by high ATP levels B. catabolic pathways are generally inhibited by high ANIP levels ® catabolic pathways are generally inhibited by high ATP levels . none of the above ATP is a “high energy” compound because A. it is an anhydride D. of phosphate transfer potential B: it is an ester E. all of the above ~C.}charge repulsion, resonance stabilization Calculate the Atkinson Energy Charge function for a cell in which [ATP] = 4.5 mM, [ADP]: 1 mM, and [AMP]=0.5 mM. The answer is i . 0.92 D. 0.67 0.83 E. 0.50 C. 0.75 Glucose labeled with 14C at C—1 is incubated with glycolytic enzymes and cofactors. Where will the label be found in pyruvate produced? A. Carbon l (carboxyl) B. Carbon 2 (keto) C Carbon 3 (methyl) In the Simpsons excerpt shown in class, what song did Bart and Lisa sing in the Karaoke Bar? (2 free points but try for correct answer) A. Gypsides, Tramps and Thieves @Theme from Shaft B. Happy Birthday E. Sakura C. Theme from Star Wars 6942301 Second Exam, page 5 Nan Part II Answer these questions on the question pages. 1. a. An enzyme is found to have a VW, at a certain concentration, of 80 mM per second. The enzyme has 3 Km of 6 mM. Calculate the initial rate when substrate itresent at 4 mM concentration. Sh03work and circle answer. Cc? {e b m : (soBC fil—i :ch\L-%%) (4) b. 0n axes provided, sketch the curves or lines which would be observed for an enzyme in the presence and absence of a noncompetitive inhibitor. The axes are for a double reciprocal ("Lineweaver Burk") plot. 0. Given that R = 1.0] and T = 1' °K, calculate the equilibrium constant for a reaction With AG" = —3.3 ol. \ {3} ACE/F: *Rru ht Cf ~33: -* (WHO) (300) in he; . —§qé,\ inkcfi jdtft/ [5’3‘oxlodLs apicefi) C 12—3—3 {90 694301 Second Exam, page 6 2. (4) (2) Narr a. Study the 2 lipids shown below. They are from different organisms. What do you know about the organisms based on the structure of these lipids? Can you deduce anything about differences between the 2 species based on what you see in the lipids shown? Diphyunyl ms b. Draw Phosphatidyl Inositol and Sphingosine. ProSpu q-L . 47/! IN) siiol 0.. JO 9 ' LI \ (a do“: 04 our} Ax‘éc CM #5- }L J r - 493 ~C§é Hz . c. Cardiohpin is afiaactefiafiipidfiht from its name it is c ear that it is found 694:301 Second Exam, page '7 Name ' 3. a. Draw Sucrose in the Haworth ring form. Q b. Membrane constituents do not generally “flip flop” from one side to the other very readily but there is a measurable rate at which they do. The fastest membrane constituents are long molecules like Coenzyme Q which span both layers. The slowest are Glycoproteins. Why would Glycoproteins be so ely slow to “flip”? (3) 0. Draw a diagram of the Acetylcholine receptor and briefly describe how it works. What does it do when stimulated? RC mli‘he re CE In S (3) Froahl ,n e c ' néS o1 9-C- View 36“. Q0 «a [c ‘N C r ' I; is fijaflérh-qu—E’ frch~ band; i \ iii % 09069 “a “CV” "3 KT (Bu-F d. What does Tetrodotoxin block? What are the symptoms of tetrodotoxin poisoning? X332) Te’rro doiox‘m blocks WW.” L L% "Act—JAG nUMb-Itj] S€;Zur6§/ \7 UH'kaLC‘Y Clea-W 69430] Second Exam, page 8 Name 4. 2:. Explain the activation of Protein Kinase A using diagrams. sfiefim fi§§§ (4) '“P‘ gag / )y (g I a e ‘ c S a b C Y ‘1 / ' c h M? 1.. ti, . V " pro-item {63 M'St Q b.* Which specific cofactors are represented below? k (4} 0 / / Nikfl \) u \ N): 0 L .0.— NH4, 3 P P——-—P f E?) PRO NAD A is F )(b Hs_C—c-——N-—{PAN19 ill—Psi”— ®w c. Fill in the cofactors where needed: _ (2) €9.56.qu Jammse 4/0 CH2 —> CH -r HO—CH -> 0=C CI-I2 ll CH2 CH2 CCOOH CH COOH COOH COOH 694:301 Second Exam, page 9 Name 5. a. Show Glycolysis starting with Glyceraldehyde—S-P and ending with Pyruvate. Draw all reactants and products, indicate cofactors, and name enzymes. 1/2 point per fact. (.33 b.* Complete the graphs below to show how kinetics of PFK-l vary first with ATP concentration and then with F6P concentration, in presence or absence of F- 2,6-BP. In other words each graph needs two lines, mark which is which. F JEFBP N0 Fla-3P I Fer —-—? ...
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This note was uploaded on 04/02/2011 for the course MOLBIO 694 taught by Professor Deis during the Spring '11 term at Rutgers.

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Second Exam - Oct 08 - Biochemistry 694:301 I\ Second Exam,...

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