lecture 6 - 9/1/10 Introductory Plant Pathology BTNY...

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Unformatted text preview: 9/1/10 Introductory Plant Pathology BTNY 301 R. D. Martyn Fall, 2010 Lecture #6 Bacteria as Plant Pathogens RD Martyn, Purdue University 1 Bacteria as Plant Pathogens RD Martyn, Purdue University 2 1 9/1/10 What are bacteria? Three Domain Model RD Martyn, Purdue University 3 Kingdoms of Life Models RD Martyn, Purdue University 4 2 9/1/10 What are bacteria? 1. _____________ (pro = before, karyon = nucelus) 2.  ___________________; single, dbl ­strand DNA ‘chromosome’ 3.  _______________________________________ 4.  _______________________________________ 5.  _______________________________________ 6.  Most have a cell wall composed of ____________________ (polymer of amino acids and sugars) 7.  Diverse morphology (___________; ___________; ___________) 8.  Divided into_______ major groups based on _________________ 9.  May be _____________________ or ____________________ 10.  Saprophytes, human, animal & plant pathogens 11.  1 ­3 ųm in size (smaller than fungi – larger than viruses) 12.  Evolved approx. 3.5 billion years ago RD Martyn, Purdue University 5 Gram Stain (Hans C. Gram – 1884) ________________ RD Martyn, Purdue University _______________ 6 3 9/1/10 A.K. Vidaver & P.A. Lambrecht hip://www.apsnet.org/educalon/IntroPlantPath/ PathogenGroups/bacteria/default.htm Bacteria as plant pathogens •  Most plant pathogenic bacteria are Gram __________ –  Agrobacterium spp. –  Erwinia spp. (Panotea spp.) –  Pseudomonas spp. (Acidovorax spp., Ralstonia spp.) –  Xanthomonas spp. •  Most are _______________________ –  Polar flagella (_________________) –  Amphitrichous flagella (________________) –  Peritrichous flagella (__________________) •  ______________________ ­ Spiroplasms RD Martyn, Purdue University 7 __________ – Pseudomonas sp. _________________ – Erwinia sp. __________________ – Agrobacterium sp. RD Martyn, Purdue University ______________ – Spiroplasmas (plants) and Spirocheates (human / animal) 8 4 9/1/10 Early history of bacteria as plant pathogens ____________________ (1839 – 1916) Professor of Botany, University of Illinois RD Martyn, Purdue University 9 Pioneering work by Thomas Burrill •  Studied the ‘pear blight disease’ (1870) – Fire blight •  Isolated bacteria from infected pears. •  Idenlfied it as Micrococcus amylovorus •  Re ­inoculated pears and reproduced ‘pear blight’ •  6 years before Robert Koch established the ‘rules of proo for pathogenicity’ in 1876. •  First to show bacteria could cause disease •  Founded the science of bacterial plant pathology RD Martyn, Purdue University 10 5 9/1/10 In Memoriam, Thomas J. Burrill Erwin F. Smith “Just as Pasteur's contribulon to science is more vital than Koch's, because it was earlier and was pioneer work, so Burrill's discovery was more difficult to make and hence more worthy of praise, than anything that has come aser. Anyone of ordinary capacity can follow a blazed trail, but only a great man can hew a path through the unbroken wilderness to be a highway for all men who come aser!” RD Martyn, Purdue University 11 ________________ 1854  ­ 1927 •  1889 – Ph.D University of Michigan (Peach yellows disease) •  Began work on bacterial plant diseases in 1890 aser M.B. Waite had developed pure culture methods for bacteria. •  First published paper was on bacterial wilt of cucurbits •  Not everyone believed bacteria caused plant diseases, especially Alfred Fischer, Germany •  Fischer – Smith debates (1890s – 1901) •  Published the first book(s) on bacteria as plant pathogens Bacterial Diseases of Plants – 4 vol. (1905, 1911, 1914, 1920) •  8th President of The American Phytopathological Society (1916) RD Martyn, Purdue University 12 6 9/1/10 ___________________ (1864 – 1945) •  Became interested in plant pathology as undergraduate when he aiended the Ph.D. exam of E. F. Smith. •  Ph.D. University of Michigan (1904) •  Worked at the Vermont Agricultural Exp. Stn. • Began working on bacterial diseases of plants during the Fischer – Smith debates. •  Showed that storage decay of carrots was caused by a bacterium which he named Erwinia carotovora. •  First to show how a pathogen (E. carotovora) caused disease – produclon of pectolylc enzymes. • Became the first president of The American Phytopathological Society (1908). RD Martyn, Purdue University 13 Plant Diseases caused by Bacteria •  _________________________ – Pectobacterium carotovorum (syn. Erwinia carotovora) •  __________________ – Erwinia amylovora) hip://www.apsnet.org/educalon/LessonsPlantPath/FireBlight/default.htm •  ___________________ – Agrobacgterium tumefaciens hip://www.apsnet.org/educalon/LessonsPlantPath/CrownGall/default.htm •  _____________________ – Xanthomonas axonopodis pv. citri hip://www.apsnet.org/educalon/LessonsPlantPath/CitrusCanker/default.htm •  ________________________– Acidovorax aveane subsp. citrulli hip://www.apsnet.org/educalon/LessonsPlantPath/BacterialBlotch/default.htm RD Martyn, Purdue University 14 7 9/1/10 Bacterial Sos Rot Pectobacterium (Erwinia) carotovora) Hosts: _________________________________ Symptoms: ___________________________________________ potato cantaloupe carrot poinseva RD Martyn, Purdue University philodendron 15 Bacterial Sos Rot Pectobacterium (Erwinia) carotovora) Cabbage Carrot Tomato Celery Onion From G.N. Agrios. 2005. Plant Pathology, 5th Ed. RD Martyn, Purdue University 16 8 9/1/10 Bacterial sos rot •  Survival –  _______________________ –  ____________________________ –  ____________________________ –  ________________________________________ •  Infeclon •  _______________ •  ________________________ •  ________________________ RD Martyn, Purdue University 17 Bacterial sos rot •  Management –  ________________________ –  ________________________ __________________________ –  _________________________ –  _________________________ –  __________________________________________ –  _________________________ –  _________________________ RD Martyn, Purdue University 18 9 ...
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This note was uploaded on 04/03/2011 for the course BTNY 301 taught by Professor Staff during the Fall '08 term at Purdue University-West Lafayette.

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