Lecture 11b - Tissue culture facilities and procedures

Lecture 11b - Tissue culture facilities and procedures -...

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Unformatted text preview: Tissue culture facilities and procedures Tissue Culture We got the lab coats, now we need a good excuse to wear them. They hate it when you carry the test tubes that way Plantlet production from an explant occurs either:- Indirectly: Callus production Callus is made first. Shoots/roots arise from callus cells.- Directly: Microcuttings No callus formed. Explant has buds. Rooted in vitro or transferred to soil to root. Micropropagation- How is it done? Micropropagation- How is it done? Callus Microcutting Explant cells dedifferentiate . Callus is a mass of undifferentiated plant cells. Balance of PGR causes cells to differentiate (shoots -> roots-> plantlets) Callus Shoots initiating indirectly from callus Micropropagation Micropropagation 1. Callus Culture: The explant has buds (produce shoots directly). Shoots are separated and rooted....
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Lecture 11b - Tissue culture facilities and procedures -...

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